Mouse Insulin-like growth factor-binding protein 5 ELISA Kit | Igfbp5 elisa kit

Mouse Insulin-like growth factor-binding protein 5 ELISA Kit

Cat. Num. AAA2883320

• Gene Names: Igfbp5; IGFBP-5; AI256729; AW208790; IGFBP-5P
• Reactivity: Mouse
• Synonyms: Insulin-like growth factor-binding protein 5; Mouse Insulin-like growth factor-binding protein 5 ELISA Kit; IBP-5; IGF-binding protein 5; IGFBP-5; Igfbp5; Igfbp-5; Igfbp5 elisa kit

• Reactivity: Mouse
• Sequence Length: 271

• Assay Type: Sandwich
• Detection Range: 0.625-40 ng/mL
• Sensitivity: 0.312 ng/mL
• Intra-Assay CV: <=6.5%
• Inter-Assay CV: <=9.3%
• Recovery: 88%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Igfbp5 elisa kit

Intended Uses||This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 70909322
• NCBI GeneID: 16011
• NCBI Accession #: NP_034648.2
• NCBI GenBank Nucleotide #: NM_010518.2
• UniProt Accession #: Q07079
• Molecular Weight: 30,372 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGFBP-5; AI256729; AW208790; IGFBP-5P
• NCBI Protein Information: insulin-like growth factor-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_MOUSE

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation

Cellular Component: extracellular region; insulin-like growth factor binding protein complex

Molecular Function: fibronectin binding; growth factor binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: alveolus development; female pregnancy; glucose homeostasis; glucose metabolic process; hair follicle morphogenesis; mammary gland involution; negative regulation of cell migration; negative regulation of growth; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of osteoblast differentiation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of translation; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of protein kinase B signaling cascade; regulation of cell growth; regulation of growth; regulation of insulin-like growth factor receptor signaling pathway; striated muscle cell differentiation