Typical Testing Data/Standard Curve (for reference only)
Rabbit Transforming Growth Factor beta3 ELISA Kit | TGFbeta3 elisa kit
Rabbit Transforming Growth Factor beta3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TGFbeta3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TGFbeta3. Standards or samples are then added to the microtiter plate wells and TGFbeta3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TGFbeta3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TGFbeta3 are added to each well to "sandwich" the TGFbeta3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGFbeta3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFbeta3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]
Uniprot Description
TGFB3: Involved in embryogenesis and cell differentiation. Homodimer; disulfide-linked. Interacts with ASPN. Belongs to the TGF-beta family.
Protein type: Cell development/differentiation; Ligand, receptor tyrosine kinase; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q24
Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; T-tubule; plasma membrane; extracellular region; nucleus
Molecular Function: identical protein binding; protein binding; growth factor activity; transforming growth factor beta binding; protein heterodimerization activity; punt binding; cytokine activity
Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; palate development; SMAD protein nuclear translocation; female pregnancy; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; platelet degranulation; mammary gland development; transforming growth factor beta receptor signaling pathway; salivary gland morphogenesis; embryonic neurocranium morphogenesis; negative regulation of neuron apoptosis; cell growth; inner ear development; aging; positive regulation of filopodium formation; uterine wall breakdown; intercellular junction assembly and maintenance; platelet activation; in utero embryonic development; positive regulation of bone mineralization; regulation of cell proliferation; positive regulation of protein secretion; gut development; response to estrogen stimulus; negative regulation of DNA replication; positive regulation of cell division; regulation of MAPKKK cascade; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; response to progesterone stimulus; alveolus development; positive regulation of DNA replication
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; Rienhoff Syndrome
Research Articles on TGFbeta3
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Product Notes
The Rabbit TGFbeta3 tgfb3 (Catalog #AAA750835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750835 ELISA Kit recognizes Rabbit TGFbeta3. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor beta3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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