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Flow Cytometry (FC/FACS) (Flow cytometry multicolor surface staining of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) PE-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension) and anti-human CD11c (BU15) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Mouse anti-Human CD206 Monoclonal Antibody | anti-CD206 antibody

Anti-Hu CD206 PE-Cyanine7

Gene Names
MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with tandem dye PE-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD206; Monoclonal Antibody; Anti-Hu CD206 PE-Cyanine7; mannose receptor C-type 1; MMR; MRC1; CLEC13DL; anti-CD206 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
15-2
Specificity
The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.
Purity/Purification
The purified antibody is conjugated with tandem dye PE-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
1456
Applicable Applications for anti-CD206 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
Purified human mannose receptor
Conjugation
PE-Cyanine7
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) PE-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension) and anti-human CD11c (BU15) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Flow cytometry multicolor surface staining of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) PE-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension) and anti-human CD11c (BU15) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)
Related Product Information for anti-CD206 antibody
CD206 (macrophage mannose receptor, MMR), also known as mannose receptor C1 (MRC1), is a type I transmembrane glycoprotein serving as pattern recognition receptor for carbogydrate groups on the surface of bacteria, fungi and other pathogens. Expressed mainly on tissue macrophages and dendritic cells, CD206 mediates endocytosis of these pathogens and presentation of their antigens to the adaptive immune system. CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
macrophage mannose receptor 1
NCBI Official Synonym Full Names
mannose receptor C-type 1
NCBI Official Symbol
MRC1
NCBI Official Synonym Symbols
MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
NCBI Protein Information
macrophage mannose receptor 1
UniProt Protein Name
Macrophage mannose receptor 1
UniProt Gene Name
MRC1
UniProt Synonym Gene Names
CLEC13D; CLEC13DL; MRC1L1; MMR
UniProt Entry Name
MRC1_HUMAN

NCBI Description

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]

Uniprot Description

MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; endosome membrane

Molecular Function: mannose binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: receptor-mediated endocytosis; signal transduction

Research Articles on CD206

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Product Notes

The CD206 mrc1 (Catalog #AAA1800053) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD206 PE-Cyanine7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD206 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD206 mrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD206, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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