Human Ribosomal Protein S19 (RPS19) ELISA Kit | RPS19 elisa kit

Human Ribosomal Protein S19 (RPS19) ELISA Kit

Cat. Num. AAA2707808

• Gene Names: RPS19; DBA; S19; DBA1; eS19; LOH19CR1
• Reactivity: Human
• Synonyms: Ribosomal Protein S19 (RPS19); Human Ribosomal Protein S19 (RPS19) ELISA Kit; DBA; Diamond-Blackfan Anemia; 40S ribosomal protein S19; RPS19 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S19 (RPS19).; No significant cross-reactivity or interference between Ribosomal Protein S19 (RPS19) and analogues was observed.
• Sequence Length: 145

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.112ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S19 (RPS19) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S19 (RPS19) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPS19 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S19 (RPS19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S19 (RPS19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S19 (RPS19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S19 (RPS19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4210954
• NCBI GeneID: 6223
• NCBI Official Full Name: ribosomal protein S19
• NCBI Official Synonym Full Names: ribosomal protein S19
• NCBI Official Symbol: RPS19
• NCBI Official Synonym Symbols: DBA; S19; DBA1; eS19; LOH19CR1
• NCBI Protein Information: 40S ribosomal protein S19
• Protein Family: Ribosomal protein

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]