Typical Testing Data/Standard Curve (for reference only)
Porcine Transforming Growth Factor beta3 ELISA Kit | TGFbeta3 elisa kit
Porcine Transforming Growth Factor beta3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TGFbeta3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGFbeta3 antibody and an TGFbeta3-HRP conjugate. The assay sample and buffer are incubated together with TGFbeta3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGFbeta3 concentration since TGFbeta3 from samples and TGFbeta3-HRP conjugate compete for the anti-TGFbeta3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TGFbeta3 from the sample, fewer sites are left to bind TGFbeta3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFbeta3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]
Uniprot Description
TGFB3: Involved in embryogenesis and cell differentiation. Homodimer; disulfide-linked. Interacts with ASPN. Belongs to the TGF-beta family.
Protein type: Ligand, receptor tyrosine kinase; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q24
Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; T-tubule; extracellular region; plasma membrane; nucleus
Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; transforming growth factor beta binding; punt binding; cytokine activity
Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; female pregnancy; palate development; SMAD protein nuclear translocation; odontogenesis; regulation of apoptosis; negative regulation of cell proliferation; platelet degranulation; mammary gland development; transforming growth factor beta receptor signaling pathway; salivary gland morphogenesis; embryonic neurocranium morphogenesis; negative regulation of neuron apoptosis; cell growth; inner ear development; aging; uterine wall breakdown; positive regulation of filopodium formation; intercellular junction assembly and maintenance; platelet activation; in utero embryonic development; positive regulation of bone mineralization; regulation of cell proliferation; positive regulation of protein secretion; gut development; negative regulation of DNA replication; response to estrogen stimulus; positive regulation of cell division; regulation of MAPKKK cascade; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; alveolus development; positive regulation of DNA replication
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; Rienhoff Syndrome
Research Articles on TGFbeta3
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Product Notes
The Porcine TGFbeta3 tgfb3 (Catalog #AAA746799) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746799 ELISA Kit recognizes Porcine TGFbeta3. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor beta3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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