Hamster Transforming Growth Factor beta2 ELISA Kit | TGFbeta2 elisa kit

Hamster Transforming Growth Factor beta2 ELISA Kit

Cat. Num. AAA17159

• Gene Names: TGFB2; LDS4; TGF-beta2
• Reactivity: Hamster
• Synonyms: Transforming Growth Factor beta2; Hamster Transforming Growth Factor beta2 ELISA Kit; TGFbeta2 elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster TGFbeta2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TGFbeta2 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster TGFbeta2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGFbeta2 and TGFbeta2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGFbeta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TGFbeta2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TGFbeta2 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster TGFbeta2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGFbeta2 and TGFbeta2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGFbeta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 208022653
• NCBI GeneID: 7042
• NCBI Accession #: NP_001129071.1
• NCBI GenBank Nucleotide #: NM_001135599.2
• UniProt Accession #: P61812; P08112; Q15579; Q15581; Q4VAV9; B4DKC5
• Molecular Weight: 47,748 Da
• NCBI Official Full Name: transforming growth factor beta-2 isoform 1
• NCBI Official Synonym Full Names: transforming growth factor, beta 2
• NCBI Official Symbol: TGFB2
• NCBI Official Synonym Symbols: LDS4; TGF-beta2
• NCBI Protein Information: transforming growth factor beta-2; G-TSF; cetermin; polyergin; BSC-1 cell growth inhibitor; glioblastoma-derived T-cell suppressor factor
• UniProt Protein Name: Transforming growth factor beta-2
• UniProt Gene Name: TGFB2
• UniProt Synonym Gene Names: TGF-beta-2; G-TSF; LAP
• UniProt Entry Name: TGFB2_HUMAN

NCBI Description

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]

Uniprot Description

TGFB2: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Belongs to the TGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: extracellular matrix; extracellular space; cell soma; axon; extracellular region; endosome

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; beta-amyloid binding; punt binding; cytokine activity; transforming growth factor beta receptor binding; receptor signaling protein serine/threonine kinase activity; receptor binding

Biological Process: heart morphogenesis; extracellular matrix organization and biogenesis; collagen fibril organization; catagen; heart development; dopamine biosynthetic process; SMAD protein nuclear translocation; protein amino acid phosphorylation; hair follicle development; cell-cell signaling; transforming growth factor beta receptor signaling pathway; somatic stem cell division; cell cycle arrest; cell growth; embryonic gut development; cartilage condensation; response to drug; neutrophil chemotaxis; platelet activation; negative regulation of immune response; neuron fate commitment; positive regulation of cell cycle; positive regulation of catagen; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cardioblast differentiation; positive regulation of protein secretion; positive regulation of cell division; activation of protein kinase activity; neuron development; response to progesterone stimulus; positive regulation of heart contraction; cell death; axon guidance; positive regulation of immune response; wound healing; cell morphogenesis; cardiac muscle cell proliferation; positive regulation of stress-activated MAPK cascade; odontogenesis; negative regulation of cell proliferation; platelet degranulation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; salivary gland morphogenesis; response to wounding; hemopoiesis; angiogenesis; positive regulation of integrin biosynthetic process; negative regulation of epithelial cell proliferation; uterine wall breakdown; intercellular junction assembly and maintenance; cell migration; regulation of transforming growth factor-beta2 production; hair follicle morphogenesis; positive regulation of cell adhesion mediated by integrin; glial cell migration; positive regulation of ossification; cell proliferation; embryonic development; eye development; generation of neurons; positive regulation of cardioblast differentiation; response to hypoxia; epithelial to mesenchymal transition; blood vessel remodeling; negative regulation of cell growth; blood coagulation

Disease: Loeys-dietz Syndrome 4