Human Transcription factor 7-like 2 ELISA Kit | TCF7L2 elisa kit

Human Transcription factor 7-like 2 ELISA Kit

Cat. Num. AAA2883541

• Gene Names: TCF7L2; TCF4; TCF-4
• Reactivity: Human
• Synonyms: Transcription factor 7-like 2; Human Transcription factor 7-like 2 ELISA Kit; HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; hTCF-4; TCF7L2; TCF4; TCF7L2 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human TCF7L2. No significant cross-reactivity or interference was observed.
• Sequence Length: 602

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 54pg/mL
• Intra-Assay CV: <=4.8%
• Inter-Assay CV: <=7.5%
• Recovery: 106%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCF7L2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human transcription factor 7-like 2,TCF7L2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TCF7L2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TCF7L2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TCF7L2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TCF7L2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226371764
• NCBI GeneID: 6934
• NCBI Accession #: NP_001139746.1
• NCBI GenBank Nucleotide #: NM_001146274.1
• UniProt Accession #: Q9NQB0; B4DRJ8; B9X074; C6ZRJ8; C6ZRK0; E2GH14; E2GH19; E2GH20; E2GH24; E2GH25; E9PFH9; F8W742
• Molecular Weight: 36,884 Da
• NCBI Official Full Name: transcription factor 7-like 2 isoform 1
• NCBI Official Synonym Full Names: transcription factor 7 like 2
• NCBI Official Symbol: TCF7L2
• NCBI Official Synonym Symbols: TCF4; TCF-4
• NCBI Protein Information: transcription factor 7-like 2
• UniProt Protein Name: Transcription factor 7-like 2
• Protein Family: Transcription factor
• UniProt Gene Name: TCF7L2
• UniProt Synonym Gene Names: TCF4; T-cell factor 4; TCF-4; hTCF-4
• UniProt Entry Name: TF7L2_HUMAN

NCBI Description

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

TCF7L2: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Belongs to the TCF/LEF family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; DNA-binding; Motility/polarity/chemotaxis; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; PML body; transcription factor complex

Molecular Function: beta-catenin binding; chromatin binding; gamma-catenin binding; nuclear hormone receptor binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: blood vessel development; bone mineralization; cell cycle arrest; cell glucose homeostasis; cell proliferation; cellular response to starvation; embryonic digestive tract morphogenesis; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis; fat cell differentiation; glucose homeostasis; glucose metabolic process; glycogen metabolic process; maintenance of DNA repeat elements; multicellular organism growth; myoblast cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of organ growth; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube development; odontogenesis of dentine-containing teeth; oligodendrocyte development; pancreas development; pituitary gland development; positive regulation of apoptosis; positive regulation of epithelial cell proliferation; positive regulation of gluconeogenesis; positive regulation of insulin secretion; positive regulation of protein binding; positive regulation of protein export from nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; regulation of hormone metabolic process; regulation of myelination; regulation of oligodendrocyte differentiation; regulation of skeletal muscle development; regulation of smooth muscle cell proliferation; regulation of transcription from RNA polymerase II promoter; response to glucose stimulus; secretory granule localization; skin development; somatic stem cell maintenance; transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin

Disease: Diabetes Mellitus, Noninsulin-dependent