Human Transcription Factor 7 Like Protein 2 (TCF7L2) ELISA Kit | TCF7L2 elisa kit

Human Transcription Factor 7 Like Protein 2 (TCF7L2) ELISA Kit

Cat. Num. AAA2088212

• Gene Names: TCF7L2; TCF4; TCF-4
• Reactivity: Human
• Synonyms: Transcription Factor 7 Like Protein 2 (TCF7L2); Human Transcription Factor 7 Like Protein 2 (TCF7L2) ELISA Kit; TCF4; HMG box transcription factor 4; TCF7L2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transcription Factor 7 Like Protein 2 (TCF7L2).; No significant cross-reactivity or interference between Transcription Factor 7 Like Protein 2 (TCF7L2) and analogues was observed.
• Sequence Length: 476

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCF7L2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor 7 Like Protein 2 (TCF7L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor 7 Like Protein 2 (TCF7L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TCF7L2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 309384250
• NCBI GeneID: 6934
• NCBI Accession #: NP_001185454.1
• NCBI GenBank Nucleotide #: NM_001198525.1
• Molecular Weight: 36,884 Da
• NCBI Official Full Name: transcription factor 7-like 2 isoform 7
• NCBI Official Synonym Full Names: transcription factor 7 like 2
• NCBI Official Symbol: TCF7L2
• NCBI Official Synonym Symbols: TCF4; TCF-4
• NCBI Protein Information: transcription factor 7-like 2
• UniProt Protein Name: Transcription factor 7-like 2
• Protein Family: Transcription factor
• UniProt Gene Name: TCF7L2
• UniProt Synonym Gene Names: TCF4; T-cell factor 4; TCF-4; hTCF-4

NCBI Description

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

TCF7L2: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Belongs to the TCF/LEF family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Cell development/differentiation; DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 10q25.2-q25.3

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; gamma-catenin binding; nuclear hormone receptor binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: blood vessel development; cell cycle arrest; cell proliferation; fat cell differentiation; glucose homeostasis; maintenance of DNA repeat elements; myoblast cell fate commitment; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pancreas development; positive regulation of insulin secretion; positive regulation of protein binding; positive regulation of protein export from nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; regulation of hormone metabolic process; regulation of smooth muscle cell proliferation; regulation of transcription from RNA polymerase II promoter; response to glucose stimulus; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Diabetes Mellitus, Noninsulin-dependent