Human Drebrin 1 (DBN1) ELISA Kit | DBN1 elisa kit

Human Drebrin 1 (DBN1) ELISA Kit

Cat. Num. AAA454138

• Gene Names: DBN1; D0S117E
• Reactivity: Human
• Synonyms: Drebrin 1 (DBN1); Human Drebrin 1 (DBN1) ELISA Kit; Developmentally-regulated brain protein; DBN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DBN1. No significant cross-reactivity or interference between DBN1 and analogues was observed.
• Sequence Length: 649

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.047ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DBN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DBN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DBN1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DBN1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DBN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DBN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DBN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18426915
• NCBI GeneID: 1627
• NCBI Accession #: NP_004386.2
• NCBI GenBank Nucleotide #: NM_004395.3
• UniProt Accession #: Q16643; Q9UFZ5; A8MV58; B2RBG0
• Molecular Weight: 76,300 Da
• NCBI Official Full Name: drebrin isoform a
• NCBI Official Synonym Full Names: drebrin 1
• NCBI Official Symbol: DBN1
• NCBI Official Synonym Symbols: D0S117E
• NCBI Protein Information: drebrin
• UniProt Protein Name: Drebrin
• Protein Family: Drebrin
• UniProt Gene Name: DBN1
• UniProt Synonym Gene Names: D0S117E

NCBI Description

The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS.