TANK recombinant protein

TANK, 1-119aa, Human, E Coli

Cat. Num. AAA203251

• Gene Names: TANK; ITRAF; TRAF2; I-TRAF
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TANK; 1-119aa; Human; E Coli; TRAF interacting protein TANK isoform b; I-TRAF; TRAF2; TRAF interacting protein TANK isoform b I TRAF; ITRAF; TRAF interacting protein; TRAF family member associated NF KAPPA B activator; TRAF family member associated NFKB activator; TRAF interacting protein TANK isoform a; TANK recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0)
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MDKNIGEQLN KAYEAFRQAC MDRDSAVKEL QQKTENYEQR IREQQEQLSL QQTIIDKLKS QLLLVNSTQD NNYGCVPLLE DSETRKNNLT LDQPQDKVIS GIAREKLPKV DIASAESSI
• Sequence Length: 119

• Applicable Applications for TANK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TANK recombinant protein

TANK, also known as TRAF (tumor necrosis factor receptor-associated Factor)-interacting protein, is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby proposing that this protein is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Overexpression of TANK inhibits TRAF2-mediated NF-Kappa-B activation signaled by CD40 and both TNF receptors and inhibits LMP1-mediated NF-kappa-B activation by blocking the association of TRAF2 with LMP1. Recombinant human TANK protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TANK recombinant protein

Cancer

References

Rothe M., et al. (1996) Proc Natl Acad Sci u S A. 93 (16):8241-6.; Gatot JS., et al. (2007) J Biol Chem. 282 (43):31131-46.;

NCBI and Uniprot Product Information

• NCBI GI #: 19743571
• NCBI GeneID: 10010
• NCBI Accession #: NP_597841
• NCBI GenBank Nucleotide #: NM_133484.1
• UniProt Accession #: Q92844; Q7Z4J6; Q92885; D3DPB5
• Molecular Weight: 13.6 kDa (119 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: TRAF family member-associated NF-kappa-B activator isoform b
• NCBI Official Synonym Full Names: TRAF family member associated NFKB activator
• NCBI Official Symbol: TANK
• NCBI Official Synonym Symbols: ITRAF; TRAF2; I-TRAF
• NCBI Protein Information: TRAF family member-associated NF-kappa-B activator
• UniProt Protein Name: TRAF family member-associated NF-kappa-B activator
• Protein Family: Tankyrase
• UniProt Gene Name: TANK
• UniProt Synonym Gene Names: ITRAF; TRAF2; I-TRAF

NCBI Description

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed:25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989). Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage (PubMed:25861989). May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2.