Western Blot (WB)
(Western blot analysis of DDX53 antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDX53 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse DDX53 Polyclonal Antibody | anti-DDX53 antibody
DDX53, CT (Probable ATP-dependent RNA Helicase DDX53, DEAD Box Protein 53, DEAD Box Protein CAGE, Cancer-associated Gene Protein, Cancer/Testis Antigen 26, CT26, CAGE) (APC)
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
DDX53; Polyclonal Antibody; CT (Probable ATP-dependent RNA Helicase DDX53; DEAD Box Protein 53; DEAD Box Protein CAGE; Cancer-associated Gene Protein; Cancer/Testis Antigen 26; CT26; CAGE) (APC); anti-DDX53 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DDX53. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
631
Applicable Applications for anti-DDX53 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human DDX53 (KLH).
Conjugate
APC
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of DDX53 antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDX53 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DDX53 antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDX53 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of WiDr cells using DDX53 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of WiDr cells using DDX53 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-DDX53 antibody
References
1. Shim, H., Lee, H. Biotechnol. Lett. 28(24), 2057-2063 (2006). 2. Cho, B., Lee, H. Biochem. Biophys. Res. Commun. 307(1), 52-63 (2003). 3. Cho, B., Lim, Y. Biochem. Biophys. Res. Commun. 292(3), 715-726 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71.154
NCBI Official Full Name
DEAD box protein 53
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX53
Protein Family
UniProt Gene Name
DDX53
UniProt Synonym Gene Names
CAGE; CT26
Uniprot Description
DDX53: This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]
Protein type: Cancer Testis Antigen (CTA); EC 3.6.4.13; Helicase
Chromosomal Location of Human Ortholog: Xp22.11
Cellular Component: cytoplasm; nucleolus
Molecular Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding
Biological Process: RNA secondary structure unwinding
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Product Notes
The DDX53 ddx53 (Catalog #AAA6472142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX53, CT (Probable ATP-dependent RNA Helicase DDX53, DEAD Box Protein 53, DEAD Box Protein CAGE, Cancer-associated Gene Protein, Cancer/Testis Antigen 26, CT26, CAGE) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DDX53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DDX53 ddx53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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