Mouse Kinase suppressor of Ras 1 ELISA Kit | Ksr1 elisa kit

Mouse Kinase suppressor of Ras 1 ELISA Kit

Cat. Num. AAA763073

• Gene Names: KSR1; KSR; RSU2
• Reactivity: Mouse
• Synonyms: Kinase suppressor of Ras 1; Mouse Kinase suppressor of Ras 1 ELISA Kit; Ksr1/Kinase suppressor of Ras 1; Ksr1 elisa kit

• Reactivity: Mouse
• Sequence Length: 762

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ksr1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ksr1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ksr1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ksr1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ksr1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 75677325
• NCBI GeneID: 8844
• NCBI Accession #: NP_055053.1
• NCBI GenBank Nucleotide #: NM_014238.1
• Molecular Weight: 84,697 Da
• NCBI Official Full Name: kinase suppressor of Ras 1
• NCBI Official Synonym Full Names: kinase suppressor of ras 1
• NCBI Official Symbol: KSR1
• NCBI Official Synonym Symbols: KSR; RSU2
• NCBI Protein Information: kinase suppressor of Ras 1
• UniProt Protein Name: Kinase suppressor of Ras 1
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR1
• UniProt Synonym Gene Names: KSR
• UniProt Entry Name: KSR1_HUMAN

Uniprot Description

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; RAF family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; protein complex

Molecular Function: ATP binding; MAP-kinase scaffold activity; metal ion binding; mitogen-activated protein kinase kinase binding; protein binding; protein C-terminus binding; protein kinase activity

Biological Process: activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway