Mouse anti-Human Interleukin-11 Monoclonal Antibody | anti-IL-11 antibody

Mouse anti Human Interleukin-11:Biotin

Cat. Num. AAA226160

• Gene Names: IL11; AGIF; IL-11
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Interleukin-11; Monoclonal Antibody; Mouse anti Human Interleukin-11:Biotin; IL-11 antibody | C16-4B8; anti-IL-11 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C16-4B8
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-IL-11 antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant human full length interleukin-11 (Pro22-Leu199)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Conjugation: Biotin
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human IL-11 using Mouse anti Human interleukin-11 as a capture reagent and biotinylated Mouse anti Human interleukin-11 (MBS226160) as a detection reagent with recombinant human IL-11 as the antigen. Detection was per)

Related Product Information for anti-IL-11 antibody

Mouse anti Human interleukin-11 antibody, clone C16-4B8, recognizes interleukin-11 (IL-11), also known as AGIF. IL-11 belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013).

The biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 as the capture antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 391353380
• NCBI GeneID: 3589
• NCBI Accession #: NP_001254647.1
• NCBI GenBank Nucleotide #: NM_001267718.1
• UniProt Accession #: P20809; Q96EB4; B4DQV5
• Molecular Weight: 21,429 Da
• NCBI Official Full Name: interleukin-11 isoform 2
• NCBI Official Synonym Full Names: interleukin 11
• NCBI Official Symbol: IL11
• NCBI Official Synonym Symbols: AGIF; IL-11
• NCBI Protein Information: interleukin-11; oprelvekin; adipogenesis inhibitory factor
• UniProt Protein Name: Interleukin-11
• Protein Family: Interleukin
• UniProt Gene Name: IL11
• UniProt Synonym Gene Names: IL-11; AGIF
• UniProt Entry Name: IL11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; cytokine activity; interleukin-11 receptor binding

Biological Process: fat cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; cell-cell signaling; megakaryocyte differentiation; B cell differentiation; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation