THRA sirna

THRA siRNA (Rat)

Cat. Num. AAA8226714

• Gene Names: Thra; ERBA1; Thra1; c-erbA-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: THRA; THRA siRNA (Rat); C-ERBA-ALPHA; NR1A1; Thyroid hormone receptor alpha; Nuclear receptor subfamily 1 group A member 1; c-erbA-1; c-erbA-alpha; THRA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: THRA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for THRA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat THRA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for THRA sirna

siRNA to inhibit THRA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 63079705
• NCBI GeneID: 81812
• NCBI Accession #: NP_001017960.1
• NCBI GenBank Nucleotide #: NM_001017960.1
• UniProt Accession #: P63059; P10685; P15827; P16416; P37241; Q63107; Q63195; Q63196; Q99146
• Molecular Weight: 46,795 Da
• NCBI Official Full Name: thyroid hormone receptor alpha isoform TRalpha1
• NCBI Official Synonym Full Names: thyroid hormone receptor alpha
• NCBI Official Symbol: Thra
• NCBI Official Synonym Symbols: ERBA1; Thra1; c-erbA-1
• NCBI Protein Information: c-erbAalpha2; thyroid hormone receptor alpha
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: Thra
• UniProt Synonym Gene Names: C-erba-alpha; Nr1a1
• UniProt Entry Name: THA_RAT

NCBI Description

binds the promoter of the Na+/H+ exchanger NHE1 and mediates thyroid hormone induced transcriptional activation [RGD, Feb 2006]

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; zinc ion binding; chromatin DNA binding; transcription factor binding; single-stranded RNA binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; learning and/or memory; thyroid gland development; erythrocyte differentiation; kidney development; cartilage condensation; response to nutrient levels; response to drug; negative regulation of transcriptional preinitiation complex assembly; ossification; cytoplasmic sequestering of transcription factor; intracellular receptor-mediated signaling pathway; adrenal gland development; negative regulation of transcription factor activity; liver development; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; female courtship behavior; gut development; embryonic organ development; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; brain development; response to cold; negative regulation of transcription, DNA-dependent; lung development