Rat Thyroid Hormone Receptor Alpha (THRa) ELISA Kit | THRa elisa kit

Rat Thyroid Hormone Receptor Alpha (THRa) ELISA Kit

Cat. Num. AAA2704304

• Gene Names: Thra; Rvr; Erba; Nr1a1; Thra1; Thra2; T3R[a]; AW259572; T3Ralpha; c-erbA-1; c-erbAalpha; c-erbA-alpha; 6430529J03Rik
• Reactivity: Rat
• Synonyms: Thyroid Hormone Receptor Alpha (THRa); Rat Thyroid Hormone Receptor Alpha (THRa) ELISA Kit; THR-A; NR1A1; AR7; EAR7; ERB-T1; ERBA1; NR1-A1; THRA1; THRA2; c-ERBA1; Erythroblastic Leukemia Viral Oncogene Homolog; Nuclear Receptor Subfamily 1; Group A; Member 1; THRa elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Alpha (THRa).; No significant cross-reactivity or interference between Thyroid Hormone Receptor Alpha (THRa) and analogues was observed.
• Sequence Length: 337

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.268ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for THRa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Alpha (THRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Alpha (THRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Alpha (THRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Hormone Receptor Alpha (THRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30017357
• NCBI GeneID: 21833
• NCBI Accession #: NP_835161.1
• NCBI GenBank Nucleotide #: NM_178060.4
• UniProt Accession #: P63058; P10685; P15827; P16416; P37241; Q542U8; Q63107; Q63195; Q63196; Q80Y90; Q99146; A3KFN4
• Molecular Weight: 37,926 Da
• NCBI Official Full Name: thyroid hormone receptor alpha isoform 2
• NCBI Official Synonym Full Names: thyroid hormone receptor alpha
• NCBI Official Symbol: Thra
• NCBI Official Synonym Symbols: Rvr; Erba; Nr1a1; Thra1; Thra2; T3R[a]; AW259572; T3Ralpha; c-erbA-1; c-erbAalpha; c-erbA-alpha; 6430529J03Rik
• NCBI Protein Information: thyroid hormone receptor alpha
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: Thra
• UniProt Synonym Gene Names: C-erba-alpha; Nr1a1

NCBI Description

The protein encoded by this gene is one of several nuclear hormone receptors that bind thyroid hormones such as triiodothyronine and thyroxine with high affinity. The encoded protein is a transcription factor that can activate or repress transcription. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015]

Uniprot Description

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Isoform Alpha-deltaE6 does not bind DNA, inhibits the activity of isoform Alpha-1, and stimulates myoblast differentiation.