Thyroid Hormone Receptor Alpha Recombinant Protein | THRa recombinant protein

Recombinant Thyroid Hormone Receptor Alpha (THRa)

Cat. Num. AAA2030199

• Gene Names: Thra; Rvr; Erba; Nr1a1; Thra1; Thra2; T3R[a]; AW259572; T3Ralpha; 6430529J03Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Thyroid Hormone Receptor Alpha; Recombinant Thyroid Hormone Receptor Alpha (THRa); THRa recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MEQKPSKVEC GSDPEENSAR SPDGKRKRKN GQCSLKTSMS GYIPSYLDKD EQCVVCGDKA TGYHYRCITC EGCKGFFRRT IQKNLHPTYS CKYDSCCVID KITRNQCQLC RFKKCIAVGM AMDLVLDDSK RVAKRKLIEQ NRERRRKEEM IRSLQQRPEP TPEEWDLIHI ATEAHRSTNA QGSHWKQRRK FLPDDIGQSP IVSMPDGDKV DLEAFSEFTK IITPAITRVV DFAKKLPMFS ELPCEDQIIL LKGCCMEIMS LRAAVRYDPE SDTLTLSGEM AVKREQLKNG GLGVVSDAIF ELGKSLSAFN LDDTEVALLQ AVLLMSTDRS GLLCVDKIEK SQEAYLLAFE HYVNHRKHNI PHFWPKLLMK EREVQSSILY KGAAAEGRPG GSLGVHPEGQ QLLGMHVVQG PQVRQLEQQL GEAGSLQGPV LQHQSPKSPQ QRLLELLHRSGILHARAVCG EDDSSEADSP SSSEEEPEVC EDLAGNAASP
• Sequence Length: 410

• Applicable Applications for THRa recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 58.5kDa
• Accurate Molecular Mass (KD): 59kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Pro490
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 30017357
• NCBI GeneID: 21833
• NCBI Accession #: NP_835161.1
• NCBI GenBank Nucleotide #: NM_178060.3
• Molecular Weight: 37,926 Da
• NCBI Official Full Name: thyroid hormone receptor alpha
• NCBI Official Synonym Full Names: thyroid hormone receptor alpha
• NCBI Official Symbol: Thra
• NCBI Official Synonym Symbols: Rvr; Erba; Nr1a1; Thra1; Thra2; T3R[a]; AW259572; T3Ralpha; 6430529J03Rik
• NCBI Protein Information: thyroid hormone receptor alpha
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: Thra
• UniProt Synonym Gene Names: C-erba-alpha; Nr1a1
• UniProt Entry Name: THA_MOUSE

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; zinc ion binding; chromatin DNA binding; transcription factor binding; single-stranded RNA binding; DNA binding; TATA-binding protein binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; learning and/or memory; thyroid gland development; erythrocyte differentiation; kidney development; cartilage condensation; response to drug; response to nutrient levels; negative regulation of transcriptional preinitiation complex assembly; ossification; cytoplasmic sequestering of transcription factor; intracellular receptor-mediated signaling pathway; adrenal gland development; negative regulation of transcription factor activity; liver development; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; female courtship behavior; gut development; embryonic organ development; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; brain development; response to cold; negative regulation of transcription, DNA-dependent; lung development