FCGR2 sirna

FCGR2 siRNA (Rat)

Cat. Num. AAA8220244

• Gene Names: Fcgr2b; CD32; FCRII; Fcgr2; FcgammaRIIB2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FCGR2; FCGR2 siRNA (Rat); FCGR2B; Low affinity immunoglobulin gamma Fc region receptor II; Fc-gamma RII; FcRII; IgG Fc receptor II beta; CD antigen CD32; FCGR2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: FCGR2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 285

• Applicable Applications for FCGR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FCGR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FCGR2 sirna

siRNA to inhibit FCGR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28461147
• NCBI GeneID: 289211
• NCBI Accession #: NP_786932.1
• NCBI GenBank Nucleotide #: NM_175756.1
• UniProt Accession #: Q63203
• Molecular Weight: 32,048 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-b
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIb, receptor
• NCBI Official Symbol: Fcgr2b
• NCBI Official Synonym Symbols: CD32; FCRII; Fcgr2; FcgammaRIIB2
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-b
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: Fcgr2
• UniProt Synonym Gene Names: Fcgr2b; FcRII
• UniProt Entry Name: FCGR2_RAT

Uniprot Description

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Oncoprotein; Membrane protein, integral; Cell surface

Cellular Component: recycling endosome; cell surface; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: IgG binding; immunoglobulin binding

Biological Process: receptor-mediated endocytosis; negative regulation of phagocytosis; negative regulation of interleukin-10 production; negative regulation of acute inflammatory response to antigenic stimulus; negative regulation of immune response; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; negative regulation of B cell proliferation; endocytic recycling; mast cell activation; negative regulation of type I hypersensitivity; response to estradiol stimulus; negative regulation of neutrophil apoptosis; negative regulation of humoral immune response mediated by circulating immunoglobulin; cell surface receptor linked signal transduction; response to bacterium; positive regulation of phagocytosis; negative regulation of immunoglobulin production; immune complex clearance; negative regulation of inflammatory response to antigenic stimulus; immune response; immunoglobulin mediated immune response; phagocytosis, engulfment