Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) Recombinant Protein | VEGFR2 recombinant protein

Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)

Cat. Num. AAA2011185

• Gene Names: Kdr; Vegfr-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); VEGFR2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- NTTLQ ITCRGQRDLD WLWPNTPRDS EERVLVTECG DSIFCKTLTV PRVVGNDTGA YKCFYRDTDV SSIVYVYVQD HRSPFIASVS DEHGIVYITE NKNKTVVIPC RGSISNLNVS LCARYPEKRF VPDGNRISWD SEKGFTIPSY MISYAGMVFC EAKINDETYQ SIMYIVLVVG YRIYDVVLSP PHEIELSAGE KLVLNCTART ELNVGLDFSW QFPSSKHQHK KIVNRDVKSL PGTVAKMFLS TLTIDSVTKS DQGEYTCTAY SGLMTKKNKT
• Sequence Length: 1343

• Applicable Applications for VEGFR2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asn46~Thr320 (Accession # O08775) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6981128
• NCBI GeneID: 25589
• NCBI Accession #: NP_037194.1
• NCBI GenBank Nucleotide #: NM_013062.1
• UniProt Accession #: O08775
• Molecular Weight: 36.8kDa
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Vegfr-2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; FLK-1; fetal liver kinase 1; kinase insert domain protein receptor; protein-tyrosine kinase receptor flk-1; FLK1 kinase insert domain receptor (VEGF receptor 2); FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2)
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk1; VEGFR-2; FLK-1
• UniProt Entry Name: VGFR2_RAT

NCBI Description

receptor for vascular endothelial growth factor (VEGF) [RGD, Feb 2006]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; TK group; VEGFR family

Cellular Component: Golgi apparatus; neuron projection; cell surface; endoplasmic reticulum; integral to plasma membrane; cytoplasmic membrane-bound vesicle; early endosome; lipid raft; cell soma; cytoplasm; plasma membrane; nucleus; cell junction; endosome; external side of plasma membrane

Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of positive chemotaxis; protein amino acid autophosphorylation; cell maturation; positive regulation of calcium-mediated signaling; positive regulation of vasodilation; positive regulation of long-term neuronal synaptic plasticity; calcium ion homeostasis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; hemopoiesis; angiogenesis; negative regulation of neuron apoptosis; vasculogenesis; positive regulation of TOR signaling pathway; positive regulation of BMP signaling pathway; aging; response to drug; endothelial cell differentiation; positive regulation of neurogenesis; cell migration; cell fate commitment; embryonic hemopoiesis; male gonad development; regulation of endothelial cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; response to hypoxia; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; surfactant homeostasis; hyaluronan metabolic process; alveolus development; positive regulation of epithelial cell proliferation; neurite morphogenesis; negative regulation of apoptosis; lung development; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process