Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) Recombinant Protein | FcgRII recombinant protein

Recombinant Receptor II For The Fc Region Of Immunoglobulin G (FcgRII)

Cat. Num. AAA2018329

• Gene Names: Fcgr2b; FCRII; Fcgr2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Receptor II For The Fc Region Of Immunoglobulin G (FcgRII); Recombinant Receptor II For The Fc Region Of Immunoglobulin G (FcgRII); FcgRII recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IQVL KEDTVTLMCE GTHNTKNCST QWFHNGSSIW HQAQANYTFK ATVNDSGEYR CRMEETGISE PIHLGVISDW LLLQTSQLVF EEGETITLRC HSWKNKQLTK VLLFQNGKPV RYYHQSSNFS IPKANHSHSG NYYCKAYLGR TMHVSKPVTI TVQEPKSSSS LPVLTIVAAV AGIAVAAIVI ILVSLVYLKKKQVPDTPSGL EEAEKNEVEN TITYSLLKHP EAPDEESDHD YQNHI
• Sequence Length: 285

• Applicable Applications for FcgRII recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ile47~Ile285 (Accession # Q63203) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 28461147
• NCBI GeneID: 289211
• NCBI Accession #: NP_786932.1
• NCBI GenBank Nucleotide #: NM_175756.1
• UniProt Accession #: Q63203
• Molecular Weight: 30.7kDa
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-b
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIb, receptor (CD32)
• NCBI Official Symbol: Fcgr2b
• NCBI Official Synonym Symbols: FCRII; Fcgr2
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-b; FC-gamma RII; igG Fc receptor II beta; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma FC region receptor II
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II
• UniProt Gene Name: Fcgr2
• UniProt Synonym Gene Names: Fcgr2b; FcRII
• UniProt Entry Name: FCGR2_RAT

Uniprot Description

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Cell surface; Oncoprotein; Membrane protein, integral

Cellular Component: recycling endosome; cell surface; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: IgG binding; immunoglobulin binding

Biological Process: receptor-mediated endocytosis; negative regulation of phagocytosis; negative regulation of interleukin-10 production; negative regulation of immune response; negative regulation of acute inflammatory response to antigenic stimulus; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; negative regulation of B cell proliferation; endocytic recycling; negative regulation of type I hypersensitivity; mast cell activation; response to estradiol stimulus; negative regulation of neutrophil apoptosis; negative regulation of humoral immune response mediated by circulating immunoglobulin; cell surface receptor linked signal transduction; response to bacterium; positive regulation of phagocytosis; negative regulation of immunoglobulin production; immune complex clearance; immune response; negative regulation of inflammatory response to antigenic stimulus; immunoglobulin mediated immune response; phagocytosis, engulfment