CASP3 sirna

CASP3 siRNA (Rat)

Cat. Num. AAA829737

• Gene Names: Casp3; Lice; Yama
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP3; CASP3 siRNA (Rat); CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; IRP; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CASP3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 277

• Applicable Applications for CASP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CASP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP3 sirna

siRNA to inhibit CASP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978605
• NCBI GeneID: 25402
• NCBI Accession #: NP_037054.1
• NCBI GenBank Nucleotide #: NM_012922.2
• UniProt Accession #: P55213; P70543; P97699; Q62993
• Molecular Weight: 31,492 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: Lice; Yama
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_RAT

NCBI Description

apoptotic cysteine-aspartic acid protease that may play a role in neuronal cell death regulation and other apoptotic processes [RGD, Feb 2006]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.56; Motility/polarity/chemotaxis; Protease; Apoptosis

Cellular Component: cytoplasm; nucleus; cytosol; lipid raft

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; phospholipase A2 activator activity; protein binding; protease binding; cysteine-type endopeptidase activity; death receptor binding; protein complex binding; aspartic-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to nicotine; positive regulation of catalytic activity; response to metal ion; wound healing; positive regulation of apoptosis; apoptosis; heart development; negative regulation of activated T cell proliferation; response to glucocorticoid stimulus; response to lipopolysaccharide; negative regulation of B cell proliferation; proteolysis; response to organic cyclic substance; response to estradiol stimulus; negative regulation of cell cycle; neuron differentiation; response to antibiotic; response to organic substance; sensory perception of sound; learning and/or memory; B cell homeostasis; positive regulation of neuron apoptosis; response to glucose stimulus; response to wounding; erythrocyte differentiation; T cell homeostasis; response to X-ray; response to UV; response to drug; release of cytochrome c from mitochondria; cell fate commitment; hippocampus development; negative regulation of cyclin-dependent protein kinase activity; response to amino acid stimulus; keratinocyte differentiation; neuron apoptosis; response to hydrogen peroxide; induction of apoptosis via death domain receptors; response to hypoxia; response to cobalt ion; protein processing; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis