Rat Caspase 3 (CASP3) ELISA Kit | CASP3 elisa kit

Rat Caspase 3 (CASP3) High Sensitive ELISA Kit

Cat. Num. AAA2087035

• Gene Names: Casp3; Lice; Yama; CPP32-beta
• Reactivity: Rat
• Synonyms: Caspase 3 (CASP3); Rat Caspase 3 (CASP3) High Sensitive ELISA Kit; CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1; CASP3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Caspase 3 (CASP3).; No significant cross-reactivity or interference between High Sensitive Caspase 3 (CASP3) and analogues was observed.
• Sequence Length: 277

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 5.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Caspase 3 (CASP3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CASP3 elisa kit

Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6978605
• NCBI GeneID: 25402
• NCBI Accession #: NP_037054.1
• NCBI GenBank Nucleotide #: NM_012922.2
• UniProt Accession #: P55213; P70543; P97699; Q62993
• Molecular Weight: 31,492 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: Lice; Yama; CPP32-beta
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1

NCBI Description

apoptotic cysteine-aspartic acid protease that may play a role in neuronal cell death regulation and other apoptotic processes [RGD, Feb 2006]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 16q11

Cellular Component: cytoplasm; cytosol; lipid raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; erythrocyte differentiation; heart development; hippocampus development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptosis; protein processing; proteolysis; response to amino acid stimulus; response to antibiotic; response to cobalt ion; response to DNA damage stimulus; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to metal ion; response to nicotine; response to organic cyclic substance; response to organic substance; response to UV; response to wounding; response to X-ray; sensory perception of sound; T cell homeostasis; wound healing