Rat Caspase-3 ELISA Kit | Casp3 elisa kit

Rat Caspase-3 ELISA Kit

Cat. Num. AAA2885847

• Gene Names: Casp3; Lice; Yama
• Reactivity: Rat
• Synonyms: Caspase-3; Rat Caspase-3 ELISA Kit; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; IRP; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1; Casp3; Cpp32; 3.4.22.56; Casp3 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Mouse Transthyretin
• Sequence Length: 277

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: <0.156 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.4%
• Inter-assay Precision: Inter-Assay CV: <=7.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Casp3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978605
• NCBI GeneID: 25402
• NCBI Accession #: NP_037054.1
• NCBI GenBank Nucleotide #: NM_012922.2
• UniProt Accession #: P55213; P70543; P97699; Q62993
• Molecular Weight: 31,492 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: Lice; Yama
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_RAT

NCBI Description

apoptotic cysteine-aspartic acid protease that may play a role in neuronal cell death regulation and other apoptotic processes [RGD, Feb 2006]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.56; Motility/polarity/chemotaxis; Protease; Apoptosis

Cellular Component: cytoplasm; cytosol; lipid raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; erythrocyte differentiation; heart development; hippocampus development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of neuron apoptosis; protein processing; proteolysis; response to amino acid stimulus; response to antibiotic; response to cobalt ion; response to DNA damage stimulus; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to metal ion; response to nicotine; response to organic cyclic substance; response to organic substance; response to UV; response to wounding; response to X-ray; sensory perception of sound; T cell homeostasis; wound healing