AQP2 sirna

AQP2 siRNA (Rat)

Cat. Num. AAA8225063

• Gene Names: Aqp2; AQP-2; aquaporin-2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AQP2; AQP2 siRNA (Rat); Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct; AQP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: AQP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 271

• Applicable Applications for AQP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AQP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AQP2 sirna

siRNA to inhibit AQP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 77539434
• NCBI GeneID: 25386
• NCBI Accession #: NP_037041.2
• NCBI GenBank Nucleotide #: NM_012909.2
• UniProt Accession #: P34080; A1A5L4
• Molecular Weight: 28,931 Da
• NCBI Official Full Name: aquaporin-2
• NCBI Official Synonym Full Names: aquaporin 2 (collecting duct)
• NCBI Official Symbol: Aqp2
• NCBI Official Synonym Symbols: AQP-2; aquaporin-2
• NCBI Protein Information: aquaporin-2
• UniProt Protein Name: Aquaporin-2
• UniProt Gene Name: Aqp2
• UniProt Synonym Gene Names: AQP-2; AQP-CD
• UniProt Entry Name: AQP2_RAT

NCBI Description

water channel protein involved in water reabsorption and vasopressin regulation in kidney collecting duct cells; mutation is linked to nephrogenic diabetes insipidous (NDI) [RGD, Feb 2006]

Uniprot Description

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Transporter; Channel, misc.; Membrane protein, integral

Cellular Component: protein complex; cytoplasmic vesicle membrane; clathrin-coated vesicle; rough endoplasmic reticulum; integral to plasma membrane; lysosome; basolateral plasma membrane; early endosome; trans-Golgi network; recycling endosome; transport vesicle; membrane; apical plasma membrane; cytoplasm; plasma membrane; cytoplasmic vesicle

Molecular Function: protein binding; water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; PDZ domain binding; glycerol channel activity

Biological Process: actin filament depolymerization; glycerol transport; response to glucagon stimulus; cellular water homeostasis; apoptosis; water transport; response to lithium ion; response to hormone stimulus; response to lipopolysaccharide; cellular response to water deprivation; female pregnancy; response to salt stress; water homeostasis; response to starvation; renal water transport; hyperosmotic response; cell volume homeostasis; positive regulation of calcium ion transport; response to calcium ion; response to water deprivation; transmembrane transport; aging