Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)
Rabbit Aquaporin 2 Polyclonal Antibody | anti-Aqp2 antibody
Aquaporin 2 Antibody
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)
Western Blot (WB)
(Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands.)
Western Blot (WB)
(Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands.)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
NCBI and Uniprot Product Information
NCBI Description
water channel protein involved in water reabsorption and vasopressin regulation in kidney collecting duct cells; mutation is linked to nephrogenic diabetes insipidous (NDI) [RGD, Feb 2006]
Uniprot Description
AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Transporter, aquaporin family; Transporter
Cellular Component: cytoplasmic vesicle membrane; protein complex; clathrin-coated vesicle; rough endoplasmic reticulum; integral to plasma membrane; lysosome; basolateral plasma membrane; early endosome; trans-Golgi network; recycling endosome; transport vesicle; membrane; cytoplasm; apical plasma membrane; plasma membrane; cytoplasmic vesicle
Molecular Function: protein binding; water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; glycerol channel activity; PDZ domain binding
Biological Process: glycerol transport; actin filament depolymerization; response to glucagon stimulus; cellular water homeostasis; apoptosis; water transport; response to lithium ion; response to hormone stimulus; response to lipopolysaccharide; cellular response to water deprivation; female pregnancy; response to salt stress; response to starvation; water homeostasis; renal water transport; hyperosmotic response; positive regulation of calcium ion transport; response to calcium ion; cell volume homeostasis; response to water deprivation; transmembrane transport; aging
Research Articles on Aqp2
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Product Notes
The Aqp2 aqp2 (Catalog #AAA805695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aquaporin 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). 1:1000-4000 (WB), 1:400 (IF). Researchers should empirically determine the suitability of the Aqp2 aqp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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