Rabbit NR0B2 / SHP Polyclonal Antibody | anti-NR0B2 antibody

Rabbit Polyclonal to Human NR0B2 / SHP

Cat. Num. AAA247534

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rabbit; Predicted Reactivity: Rat, Bat, Bovine (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NR0B2 / SHP; Polyclonal Antibody; Rabbit Polyclonal to Human NR0B2 / SHP; Anti-NR0B2 / SHP Antibody (Ligand-binding Domain) IHC-plus; NR0B2; Short heterodimer partner; SHP; Nuclear receptor SHP; Orphan nuclear receptor SHP; Small heterodimer partner; SHP1; Human NR0B2; anti-NR0B2 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rabbit; Predicted Reactivity: Rat, Bat, Bovine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 257

• Applicable Applications for anti-NR0B2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (4 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%); Rat, Bovine, Bat (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: NR0B2 / SHP antibody was raised against synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%); Rat, Bovine, Bat (94%); Elephant, Panda, Horse, Pig, Opossum (88%); Dog (81%).
• Antigen Modification: Ligand-binding Domain
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-NR0B2 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-NR0B2 antibody

SHP (short heterodimer partner) is a bile acid-dependent orphan NR0 Knirps-Like receptor with dimerization and ligand-binding domains but not the conventional DNA-binding domain. SHP has been shown to inhibit the transcriptional activity of other nuclear receptors, including thyroid hormone receptor, constitutive androstane receptor, and retinoic acid receptors.

Product Categories/Family for anti-NR0B2 antibody

Family: NHR; Subfamily: NR0 Knirps-like

NCBI and Uniprot Product Information

• NCBI GI #: 13259503
• NCBI GeneID: 8431
• NCBI Accession #: NP_068804.1
• NCBI GenBank Nucleotide #: NM_021969.2
• UniProt Accession #: Q15466; Q5QP36; F1D8P5
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP
• UniProt Entry Name: NR0B2_HUMAN

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Nuclear receptor

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein domain specific binding; protein binding; peroxisome proliferator activated receptor binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription corepressor activity; transcription factor activity

Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter

Disease: Obesity