Typical Testing Data/Standard Curve (for reference only)
Rat Aquaporin-2 ELISA Kit | Aqp2 elisa kit
Rat Aquaporin-2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
water channel protein involved in water reabsorption and vasopressin regulation in kidney collecting duct cells; mutation is linked to nephrogenic diabetes insipidous (NDI) [RGD, Feb 2006]
Uniprot Description
AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Transporter, aquaporin family; Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: apical plasma membrane; basolateral plasma membrane; clathrin-coated vesicle; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; early endosome; integral to membrane; lysosome; membrane; plasma membrane; protein complex; recycling endosome; rough endoplasmic reticulum; trans-Golgi network; transport vesicle
Molecular Function: actin binding; glycerol channel activity; glycerol transmembrane transporter activity; PDZ domain binding; protein binding; water channel activity; water transporter activity
Biological Process: actin filament depolymerization; aging; apoptosis; cell volume homeostasis; cellular response to water deprivation; female pregnancy; glycerol transport; hyperosmotic response; positive regulation of calcium ion transport; renal water transport; response to calcium ion; response to glucagon stimulus; response to hormone stimulus; response to lipopolysaccharide; response to lithium ion; response to salt stress; response to starvation; response to water deprivation; water homeostasis; water transport
Research Articles on Aqp2
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Product Notes
The Rat Aqp2 aqp2 (Catalog #AAA289561) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289561 ELISA Kit recognizes Rat Aqp2. It is sometimes possible for the material contained within the vial of "Aquaporin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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