TIRAP sirna

TIRAP siRNA (Mouse)

Cat. Num. AAA8229321

• Gene Names: Tirap; Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TIRAP; TIRAP siRNA (Mouse); MAL; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; TIRAP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TIRAP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 249

• Applicable Applications for TIRAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TIRAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TIRAP sirna

siRNA to inhibit TIRAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295390024
• NCBI GeneID: 117149
• NCBI Accession #: NP_473437.2
• NCBI GenBank Nucleotide #: NM_054096.2
• UniProt Accession #: Q99JY1; Q91ZW0
• Molecular Weight: 26,035 Da
• NCBI Official Full Name: toll/interleukin-1 receptor domain-containing adapter protein
• NCBI Official Synonym Full Names: toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
• NCBI Official Symbol: Tirap
• NCBI Official Synonym Symbols: Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
• NCBI Protein Information: toll/interleukin-1 receptor domain-containing adapter protein
• UniProt Protein Name: Toll/interleukin-1 receptor domain-containing adapter protein
• Protein Family: Toll/interleukin-1 receptor domain-containing adapter protein
• UniProt Gene Name: Tirap
• UniProt Synonym Gene Names: Mal; TIR domain-containing adapter protein
• UniProt Entry Name: TIRAP_MOUSE

Uniprot Description

TIRAP: an adapter protein involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, ERK1, ERK2 and JNK, resulting in cytokine secretion and the inflammatory response. Homodimerizes. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9. Genetic variations in TIRAP can influence susceptibility or resistance to infectious diseases, including invasive pneumococcal disease, malaria and tuberculosis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; intercellular bridge; membrane; endocytic vesicle; cytoplasm; plasma membrane; peroxisome; intracellular

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase C binding; protein heterodimerization activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; response to lipopolysaccharide; signal transduction; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; cell surface receptor linked signal transduction; positive regulation of interleukin-15 production; positive regulation of innate immune response; positive regulation of B cell proliferation; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; immune system process; positive regulation of toll-like receptor 4 signaling pathway; regulation of interleukin-15 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor 2 signaling pathway; regulation of innate immune response; defense response to Gram-positive bacterium; response to bacterium; innate immune response; myeloid cell differentiation; regulation of interferon-beta production