TAP2 sirna

TAP2 siRNA (Mouse)

Cat. Num. AAA8215942

• Gene Names: Tap2; Y1; jas; APT2; Ham2; MTP2; PSF2; ABC18; Abcb3; Ham-2; Tap-2; RING11; AI462429
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAP2; TAP2 siRNA (Mouse); ABCB3; HAM-2; HAM2; Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; Histocompatibility antigen modifier 2; TAP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TAP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 702

• Applicable Applications for TAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAP2 sirna

siRNA to inhibit TAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239915963
• NCBI GeneID: 21355
• NCBI Accession #: NP_035660.3
• NCBI GenBank Nucleotide #: NM_011530.3
• UniProt Accession #: P36371
• Molecular Weight: 77,445 Da
• NCBI Official Full Name: antigen peptide transporter 2
• NCBI Official Synonym Full Names: transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
• NCBI Official Symbol: Tap2
• NCBI Official Synonym Symbols: Y1; jas; APT2; Ham2; MTP2; PSF2; ABC18; Abcb3; Ham-2; Tap-2; RING11; AI462429
• NCBI Protein Information: antigen peptide transporter 2
• UniProt Protein Name: Antigen peptide transporter 2
• Protein Family: Protein
• UniProt Gene Name: Tap2
• UniProt Synonym Gene Names: Abcb3; Ham-2; Ham2; APT2
• UniProt Entry Name: TAP2_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with Tap1 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. [provided by RefSeq, Jul 2008]

Uniprot Description

TAP2: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Transports peptides across the endoplasmic reticulum membrane for assembly of major histocompatibility complex class I molecules. Two subunits, TAP1 and TAP2, are required for peptide transport. ATP hydrolysis is important for transport activity. Nascent MHC class I molecules associate with TAP via tapasin.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter

Cellular Component: TAP complex; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; extracellular region; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein homodimerization activity; peptide transporter activity; TAP1 binding; peptide antigen binding; ATPase activity; nucleotide binding; ADP binding; tapasin binding; protein binding; ATPase activity, coupled to transmembrane movement of substances; protein heterodimerization activity; MHC class I protein binding; TAP2 binding; peptide antigen-transporting ATPase activity; MHC protein binding; ATP binding; peptide-transporting ATPase activity

Biological Process: peptide transport; intracellular transport of viral proteins in host cell; positive regulation of T cell mediated cytotoxicity; immune system process; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; protection from natural killer cell mediated cytotoxicity; cytosol to ER transport; protein transport; transport; response to molecule of bacterial origin; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; peptide antigen transport; protein complex assembly; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent