Human Antigen peptide transporter 2 (TAP2) ELISA Kit | TAP2 elisa kit

Human Antigen peptide transporter 2 (TAP2) ELISA Kit

Cat. Num. AAA7253328

• Gene Names: TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
• Reactivity: Human
• Synonyms: Antigen peptide transporter 2 (TAP2); Human Antigen peptide transporter 2 (TAP2) ELISA Kit; TAP2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAP2 elisa kit

Intended Uses: This TAP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TAP2.

Principle of the Assay: TAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-TAP2 antibody and an TAP2-HRP conjugate. The assay sample and buffer are incubated together with TAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAP2 concentration since TAP2 from samples and TAP2-HRP conjugate compete for the anti-TAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TAP2 from the sample, fewer sites are left to bind TAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAP2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TAP2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 73747915
• NCBI GeneID: 6891
• NCBI Accession #: NP_000535.3
• NCBI GenBank Nucleotide #: NM_000544.3
• Molecular Weight: 72,003 Da
• NCBI Official Full Name: antigen peptide transporter 2 isoform 1
• NCBI Official Synonym Full Names: transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
• NCBI Official Symbol: TAP2
• NCBI Official Synonym Symbols: APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
• NCBI Protein Information: antigen peptide transporter 2; ABC transporter, MHC 2; peptide supply factor 2; peptide transporter PSF2; really interesting new gene 11 protein; transporter 2, ABC (ATP binding cassette); peptide transporter involved in antigen processing 2; ATP-binding cassette, sub-family B (MDR/TAP), member 3
• UniProt Protein Name: Antigen peptide transporter 2
• Protein Family: Antigen peptide transporter
• UniProt Gene Name: TAP2
• UniProt Synonym Gene Names: ABCB3; PSF2; RING11; Y1; APT2; PSF-2
• UniProt Entry Name: TAP2_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]

Uniprot Description

TAP2: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Transports peptides across the endoplasmic reticulum membrane for assembly of major histocompatibility complex class I molecules. Two subunits, TAP1 and TAP2, are required for peptide transport. ATP hydrolysis is important for transport activity. Nascent MHC class I molecules associate with TAP via tapasin.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; TAP complex; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: tapasin binding; protein binding; peptide transporter activity; TAP1 binding; transporter activity; peptide antigen-transporting ATPase activity; ATP binding

Biological Process: intracellular transport of viral proteins in host cell; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I; cytosol to ER transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen transport; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Bare Lymphocyte Syndrome, Type I