Rabbit anti-Mouse FBXO8 Polyclonal Antibody

FBXO8, ID (FBXO8, FBS, FBX8, F-box only protein 8, F-box/SEC7 protein FBS)

Cat. Num. AAA643946

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FBXO8; Polyclonal Antibody; ID (FBXO8; FBS; FBX8; F-box only protein 8; F-box/SEC7 protein FBS); Anti -FBXO8; anti-FBXO8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-FBXO8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: FBXO8 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 167-195 amino acids selected from the Central region of human FBXO8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO8 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the FBXO8 antibody detected the FBXO8 protein (arrow).)

Related Product Information for anti-FBXO8 antibody

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity.

Product Categories/Family for anti-FBXO8 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 37182563
• NCBI Official Full Name: FBXO8
• Protein Family: F-box only protein

Uniprot Description

FBXO8: an F-box protein that apparently mediates the ubiquitination of Arf6. May function as a guanine nucleotide exchange factor (GEF) that activates ARF G proteins. The loss of FBXO8 correlates with poor survival in hepatocellular carcinoma.

Protein type: Ubiquitin conjugating system; GEFs; GEFs, ARF

Chromosomal Location of Human Ortholog: 4q34.1

Cellular Component: trans-Golgi network; nucleus; cell junction; ubiquitin ligase complex

Molecular Function: ARF guanyl-nucleotide exchange factor activity

Biological Process: ubiquitin-dependent protein catabolic process; vesicle-mediated transport; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity