SHANK3 sirna

SHANK3 siRNA (Mouse)

Cat. Num. AAA8225884

• Gene Names: Shank3; Shank3b; AI841104; SHANK3c-3; SHANK3c-4
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SHANK3; SHANK3 siRNA (Mouse); KIAA1650; PROSAP2; SH3 and multiple ankyrin repeat domains protein 3; Shank3; Proline-rich synapse-associated protein 2; ProSAP2; SPANK-2; SHANK3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SHANK3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 840

• Applicable Applications for SHANK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SHANK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SHANK3 sirna

siRNA to inhibit SHANK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71896543
• NCBI GeneID: 58234
• NCBI Accession #: NP_067398.2
• NCBI GenBank Nucleotide #: NM_021423.3
• UniProt Accession #: Q4ACU6; Q69ZD8; Q9JJZ3; F8S0X0; F8S0X2; F8S0X3; F8S0X5; F8S0X6; S6BMD3; S6CCV8
• Molecular Weight: 88,196 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 3
• NCBI Official Synonym Full Names: SH3/ankyrin domain gene 3
• NCBI Official Symbol: Shank3
• NCBI Official Synonym Symbols: Shank3b; AI841104; SHANK3c-3; SHANK3c-4
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 3
• UniProt Protein Name: SH3 and multiple ankyrin repeat domains protein 3
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: Shank3
• UniProt Synonym Gene Names: Kiaa1650; Prosap2; Shank3; ProSAP2
• UniProt Entry Name: SHAN3_MOUSE

Uniprot Description

SHANK3: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B. Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome). Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15). SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; neuron projection; cell projection; membrane; cell; cytoplasm; postsynaptic density; plasma membrane; excitatory synapse; synapse; intracellular; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; ionotropic glutamate receptor binding; protein binding; protein self-association; zinc ion binding; GKAP/Homer scaffold activity; actin binding; SH3 domain binding

Biological Process: negative regulation of actin filament bundle formation; MAPKKK cascade; vocal learning; social behavior; learning; positive regulation of synaptic transmission, glutamatergic; positive regulation of long-term neuronal synaptic plasticity; protein oligomerization; memory; positive regulation of synapse structural plasticity; synaptogenesis; striatal medium spiny neuron differentiation; adult behavior; brain morphogenesis; embryonic epithelial tube formation; neuromuscular process controlling balance; negative regulation of cell volume