Western Blot (WB)
(All lanes : Anti-SFTPC Antibody (N-term) at 1:2000 dilutionLane 1: human lung lysatesLane 2: rat lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit SFTPC Polyclonal Antibody | anti-SFTPC antibody
SFTPC Antibody (N-term)
IF~~1:25
Western Blot (WB)
(All lanes : Anti-SFTPC Antibody (N-term) at 1:2000 dilutionLane 1: human lung lysatesLane 2: rat lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SFTPC Antibody (N-term) at 1:2000 dilutionLane 1: human lung lysatesLane 2: rat lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Human lung tissues, using SFTPC Antibody (N-term). MBS9206337 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Human lung tissues, using SFTPC Antibody (N-term). MBS9206337 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(SFTPC Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the SFTPC antibody detected the SFTPC protein (arrow).)
Western Blot (WB)
(SFTPC Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the SFTPC antibody detected the SFTPC protein (arrow).)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Thouvenin, G., et al. Arch. Dis. Child. 95(6):449-454(2010)
Crossno, P.F., et al. Chest 137(4):969-973(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]
Uniprot Description
SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: multivesicular body; extracellular space
Molecular Function: protein binding; protein homodimerization activity
Biological Process: circadian rhythm; response to cAMP; response to hyperoxia; response to retinoic acid; response to glucocorticoid stimulus; response to glucose stimulus; response to lipopolysaccharide; respiratory gaseous exchange; protein homooligomerization; response to vitamin A
Disease: Pulmonary Fibrosis, Idiopathic; Surfactant Metabolism Dysfunction, Pulmonary, 2
Research Articles on SFTPC
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Product Notes
The SFTPC sftpc (Catalog #AAA9206337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The SFTPC Antibody (N-term) reacts with Human, mouse, rat (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's SFTPC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000 IF~~1:25. Researchers should empirically determine the suitability of the SFTPC sftpc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFTPC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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