MAPKAP1 sirna

MAPKAP1 siRNA (Mouse)

Cat. Num. AAA8235822

• Gene Names: Mapkap1; Sin1; AI591529; D230039K05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPKAP1; MAPKAP1 siRNA (Mouse); MIP1; SIN1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1; Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; MAPKAP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAPKAP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 522

• Applicable Applications for MAPKAP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAPKAP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPKAP1 sirna

siRNA to inhibit MAPKAP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 594591548
• NCBI GeneID: 227743
• NCBI Accession #: NP_001277554.1
• NCBI GenBank Nucleotide #: NM_001290625.1
• UniProt Accession #: Q8BKH7; Q80UY4; Q8BMV5; Q8R2N9; A2AN72; A2AN74; A2AR13; A2AR16
• Molecular Weight: 37,303 Da
• NCBI Official Full Name: target of rapamycin complex 2 subunit MAPKAP1 isoform a
• NCBI Official Synonym Full Names: mitogen-activated protein kinase associated protein 1
• NCBI Official Symbol: Mapkap1
• NCBI Official Synonym Symbols: Sin1; AI591529; D230039K05Rik
• NCBI Protein Information: target of rapamycin complex 2 subunit MAPKAP1
• UniProt Protein Name: Target of rapamycin complex 2 subunit MAPKAP1
• Protein Family: Target of rapamycin complex
• UniProt Gene Name: Mapkap1
• UniProt Synonym Gene Names: Mip1; Sin1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1
• UniProt Entry Name: SIN1_MOUSE

Uniprot Description

Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding

Biological Process: negative regulation of Ras protein signal transduction