GART sirna

GART siRNA (Mouse)

Cat. Num. AAA8239607

• Gene Names: Gart; Gaps; Prgs
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GART; GART siRNA (Mouse); Trifunctional purine biosynthetic protein adenosine-3; GART sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GART siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1010

• Applicable Applications for GART sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GART gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GART sirna

siRNA to inhibit GART expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 93102415
• NCBI GeneID: 14450
• NCBI Accession #: NP_034386.2
• NCBI GenBank Nucleotide #: NM_010256.2
• UniProt Accession #: Q64737; Q3TGI3; Q6NS48
• Molecular Weight: 45,698 Da
• NCBI Official Full Name: trifunctional purine biosynthetic protein adenosine-3
• NCBI Official Synonym Full Names: phosphoribosylglycinamide formyltransferase
• NCBI Official Symbol: Gart
• NCBI Official Synonym Symbols: Gaps; Prgs
• NCBI Protein Information: trifunctional purine biosynthetic protein adenosine-3
• UniProt Protein Name: Trifunctional purine biosynthetic protein adenosine-3
• UniProt Gene Name: Gart
• UniProt Synonym Gene Names: GARS; AIRS; GART
• UniProt Entry Name: PUR2_MOUSE

Uniprot Description

GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described.

Protein type: Mitochondrial; EC 6.3.4.13; Methyltransferase; Cofactor and Vitamin Metabolism - one carbon pool by folate; Ligase; EC 6.3.3.1; Nucleotide Metabolism - purine; EC 2.1.2.2; Enzyme, cellular metabolism

Cellular Component: cytoplasm

Molecular Function: transferase activity; methyltransferase activity; phosphoribosylamine-glycine ligase activity; metal ion binding; nucleotide binding; hydroxymethyl-, formyl- and related transferase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity; catalytic activity; ATP binding; ligase activity

Biological Process: response to organic substance; glycine metabolic process; tetrahydrofolate biosynthetic process; response to inorganic substance; purine base biosynthetic process; metabolic process; ribonucleoside monophosphate biosynthetic process; biosynthetic process; purine nucleotide biosynthetic process; 'de novo' IMP biosynthetic process