Rabbit OPN1MW Polyclonal Antibody | anti-OPN1MW antibody

OPN1MW Antibody (N-term)

Cat. Num. AAA9203278

• Gene Names: OPN1MW; CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• Reactivity: Human; Predicted: Rat, Chicken, Xenopus
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: OPN1MW; Polyclonal Antibody; OPN1MW Antibody (N-term); Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin; GOP; GCP; anti-OPN1MW antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Rat, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This OPN1MW antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human OPN1MW.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 21-50
• Sequence Length: 364

• Applicable Applications for anti-OPN1MW antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10-50
• Antigen Type: Synthetic Peptide
• Function: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
• Cellular Location: Cell membrane; Multi-pass membrane protein
• Tissue Location: The three color pigments are found in the cone photoreceptor cells.
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of OPN1MW Antibody (N-term) in HepG2 cell line lysates (35ug/lane). OPN1MW (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(OPN1MW Antibody (N-term) (MBS9203278) flow cytometric analysis of HepG2 K10cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Anti-OPN1MW Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-OPN1MW antibody

OPN1MW encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness.

Product Categories/Family for anti-OPN1MW antibody

Neuroscience; Signal Transduction; GPCR Antibodies

References

Thirumuruganandham, S.P., et al. J Mol Model 15(8):959-969(2009)
Ala-Laurila, P., et al. J. Biol. Chem. 284(24):16492-16500(2009)
Holmes, M.V., et al. PLoS ONE 4 (12), E7960 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4503965
• NCBI GeneID: 2652
• NCBI Accession #: NP_000504.1; NP_001041646.1
• NCBI GenBank Nucleotide #: NM_000513.2
• UniProt Accession #: P04001
• Molecular Weight: 40584
• NCBI Official Full Name: medium-wave-sensitive opsin 1
• NCBI Official Synonym Full Names: opsin 1 (cone pigments), medium-wave-sensitive
• NCBI Official Symbol: OPN1MW
• NCBI Official Synonym Symbols: CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• NCBI Protein Information: medium-wave-sensitive opsin 1
• UniProt Protein Name: Medium-wave-sensitive opsin 1
• Protein Family: Medium-wave-sensitive opsin
• UniProt Gene Name: OPN1MW
• UniProt Synonym Gene Names: GCP; GOP
• UniProt Entry Name: OPSG_HUMAN

NCBI Description

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness. [provided by RefSeq, Mar 2009]

Uniprot Description

OPN1MW: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Defects in OPN1MW are the cause of partial colorblindness deutan series (CBD); also known as deuteranopia. Defects in OPN1MW are a cause of blue cone monochromacy (BCM). A rare X-linked congenital stationary cone dysfunction syndrome characterized by the absence of functional long wavelength-sensitive and medium wavelength-sensitive cones in the retina. Color discrimination is severely impaired from birth, and vision is derived from the remaining preserved blue (S) cones and rod photoreceptors. BCM typically presents with reduced visual acuity, pendular nystagmus, and photophobia. Patients often have myopia. Defects in OPN1MW are a cause of cone dystrophy type 5 (COD5). A X-linked cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; photoreceptor activity

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; phototransduction, visible light; visual perception; retinoid metabolic process; protein-chromophore linkage

Disease: Blue Cone Monochromacy; Colorblindness, Partial, Deutan Series