Rabbit anti-Human APH1 Polyclonal Antibody | anti-APH1 antibody

APH1, NT (Gamma-secretase Subunit APH-1A, APH-1a, APH-1a, Aph-1alpha, Presenilin-stabilization Factor, PSF, CGI-78, UNQ579/PRO1141) (AP)

Cat. Num. AAA6463307

• Gene Names: APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: APH1; Polyclonal Antibody; NT (Gamma-secretase Subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization Factor; PSF; CGI-78; UNQ579/PRO1141) (AP); anti-APH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APH1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 265

• Applicable Applications for anti-APH1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human APH1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-APH1 Antibody (N-term) in A2058 cell line lysates (35ug/lane). APH1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-APH1 antibody

Antibodies; Membrane Proteins

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 3. Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003). 4. Kimberly, W.T., et al., Proc. Natl. Acad. Sci. USA 100(11):6382-6387 (2003). 5. Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 117606362
• NCBI GeneID: 51107
• NCBI Accession #: NP_001071096; NP_057106
• NCBI GenBank Nucleotide #: NP_001071096.1
• UniProt Accession #: Q96BI3
• Molecular Weight: 28.996
• NCBI Official Full Name: gamma-secretase subunit APH-1A isoform 1
• NCBI Official Synonym Full Names: aph-1 homolog A, gamma-secretase subunit
• NCBI Official Symbol: APH1A
• NCBI Official Synonym Symbols: APH-1; APH-1A; CGI-78; 6530402N02Rik
• NCBI Protein Information: gamma-secretase subunit APH-1A
• UniProt Protein Name: Gamma-secretase subunit APH-1A
• Protein Family: Gamma-secretase
• UniProt Gene Name: APH1A
• UniProt Synonym Gene Names: PSF; APH-1a
• UniProt Entry Name: APH1A_HUMAN

NCBI Description

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

APH1A: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.13-q31.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; Notch signaling pathway; amyloid precursor protein catabolic process; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; ephrin receptor signaling pathway; Notch receptor processing; protein processing; metanephros development