Western Blot (WB)
(Anti-GART Antibody (C-Term) at 1:2000 dilution + mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse GART Polyclonal Antibody | anti-GART antibody
GART Antibody (C-Term)
Western Blot (WB)
(Anti-GART Antibody (C-Term) at 1:2000 dilution + mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GART Antibody (C-Term) at 1:2000 dilution + mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described.
Protein type: EC 2.1.2.2; EC 6.3.4.13; Cofactor and Vitamin Metabolism - one carbon pool by folate; Nucleotide Metabolism - purine; Methyltransferase; Ligase; Mitochondrial; Enzyme, cellular metabolism; EC 6.3.3.1
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: cytosol
Molecular Function: ATP binding; metal ion binding; phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity
Biological Process: 'de novo' IMP biosynthetic process; cerebellum development; cerebral cortex development; glycine metabolic process; purine base biosynthetic process; purine ribonucleoside monophosphate biosynthetic process; response to inorganic substance; response to organic substance; tetrahydrofolate biosynthetic process
Research Articles on GART
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Product Notes
The GART gart (Catalog #AAA9217242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GART Antibody (C-Term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GART can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the GART gart for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GART, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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