Typical Testing Data/Standard Curve (for reference only)
Human beta sarcoglycan (SGCB) ELISA Kit | SGCB elisa kit
Human beta sarcoglycan (SGCB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SGCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SGCB antibody and an SGCB-HRP conjugate. The assay sample and buffer are incubated together with SGCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SGCB concentration since SGCB from samples and SGCB-HRP conjugate compete for the anti-SGCB antibody binding site. Since the number of sites is limited, as more sites are occupied by SGCB from the sample, fewer sites are left to bind SGCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SGCB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy.[provided by RefSeq, Oct 2008]
Uniprot Description
SGCB: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCB are the cause of limb-girdle muscular dystrophy type 2E (LGMD2E). LGMD2E is an autosomal recessive disorder. Belongs to the sarcoglycan beta/delta/gamma/zeta family.
Protein type: Dystrophin complex; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q12
Cellular Component: dystrophin-associated glycoprotein complex; cytoskeleton; integral to plasma membrane; cytoplasm; sarcoglycan complex; sarcolemma
Biological Process: muscle development; cardiac muscle cell development; muscle fiber development
Disease: Muscular Dystrophy, Limb-girdle, Type 2e
Research Articles on SGCB
Similar Products
Product Notes
The Human SGCB sgcb (Catalog #AAA7233681) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233681 ELISA Kit recognizes Human SGCB. It is sometimes possible for the material contained within the vial of "beta sarcoglycan (SGCB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
