FGD1 sirna

FGD1 siRNA (Mouse)

Cat. Num. AAA8205441

• Gene Names: Fgd1; ZFYVE3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FGD1; FGD1 siRNA (Mouse); FYVE. RhoGEF and PH domain-containing protein 1; Faciogenital dysplasia 1 protein homolog; Rho/Rac guanine nucleotide exchange factor FGD1; Rho/Rac GEF; Zinc finger FYVE domain-containing protein 3; FGD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FGD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 960

• Applicable Applications for FGD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FGD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FGD1 sirna

siRNA to inhibit FGD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31982367
• NCBI GeneID: 14163
• NCBI Accession #: NP_032027.2
• NCBI GenBank Nucleotide #: NM_008001.4
• UniProt Accession #: P52734; Q921L2
• Molecular Weight: 106,365 Da
• NCBI Official Full Name: FYVE, RhoGEF and PH domain-containing protein 1
• NCBI Official Synonym Full Names: FYVE, RhoGEF and PH domain containing 1
• NCBI Official Symbol: Fgd1
• NCBI Official Synonym Symbols: ZFYVE3
• NCBI Protein Information: FYVE, RhoGEF and PH domain-containing protein 1
• UniProt Protein Name: FYVE, RhoGEF and PH domain-containing protein 1
• Protein Family: FYVE, RhoGEF and PH domain-containing protein
• UniProt Gene Name: Fgd1
• UniProt Synonym Gene Names: Rho/Rac GEF
• UniProt Entry Name: FGD1_MOUSE

NCBI Description

This gene encodes a member of family of Rho-specific guanine nucleotide exchange factors. Rho-specific guanine nucleotide exchange factors catalyze the exchange of GDP for GTP and activate small GTPases, which function as molecular switches in signaling. This protein specifically binds cell division cycle 42, a Rho (Ras homology) GTPase. Investigations in mouse suggest that this protein is important for skeletal mineralization and for regulating the actin cytoskeleton. In humans, mutations in this gene are associated with faciogenital dysplasia, also known as Aarskog-Scott syndrome. [provided by RefSeq, Mar 2014]

Uniprot Description

FGD1: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Interacts with DBNL/ABP1 and CTTN. May interact with CCPG1. Binds CDC42. Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle.

Protein type: GEFs, misc.; GEFs

Cellular Component: ruffle; Golgi apparatus; cell projection; cytoskeleton; lamellipodium; cytoplasm

Molecular Function: small GTPase binding; protein binding; Rho guanyl-nucleotide exchange factor activity; metal ion binding; guanyl-nucleotide exchange factor activity

Biological Process: regulation of cell shape; filopodium formation; regulation of Rho protein signal transduction; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity