Rabbit anti-Human ULK1 Polyclonal Antibody | anti-ULK1 antibody

ULK1, ID (S317) (ULK1, KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1) (Biotin)

Cat. Num. AAA6361291

• Gene Names: ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ULK1; Polyclonal Antibody; ID (S317) (ULK1; KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1) (Biotin); Unc-51-like kinase 1; anti-ULK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ULK1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ULK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 296-322 in the central region of human ULK1, Swiss/UniProt Accession: O75385.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6005748. This demonstrates that MBS6005748 detected the ULK1 protein (arrow).)

Related Product Information for anti-ULK1 antibody

ULK1 is involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells (By similarity).

Product Categories/Family for anti-ULK1 antibody

Antibodies; Protein Kinases

References

1. Mercer, C.A., et al. Autophagy 5(5):649-662(2009). 2. Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009). 3. Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009). 4. Hosokawa, N., et al. Mol. Biol. Cell 20(7):1981-1991(2009). 5. Chan, E.Y. Sci Signal 2 (84), PE51 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507831
• NCBI GeneID: 8408
• NCBI Accession #: NP_003556.1
• NCBI GenBank Nucleotide #: NM_003565.2
• UniProt Accession #: O75385; Q9UQ28
• Molecular Weight: 112631 MW
• NCBI Official Full Name: serine/threonine-protein kinase ULK1
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 1
• NCBI Official Symbol: ULK1
• NCBI Official Synonym Symbols: ATG1; ATG1A; UNC51; hATG1; Unc51.1
• NCBI Protein Information: serine/threonine-protein kinase ULK1
• UniProt Protein Name: Serine/threonine-protein kinase ULK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK1
• UniProt Synonym Gene Names: KIAA0722; ATG1; hATG1
• UniProt Entry Name: ULK1_HUMAN

Uniprot Description

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; Other group; ULK family

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; ATP binding; Rab GTPase binding

Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; cellular response to nutrient levels; protein localization; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; positive regulation of autophagy; neurite development; autophagic vacuole formation