Rabbit anti-Human Leo1-S151 Polyclonal Antibody | anti-LEO1 antibody

Leo1-S151 Antibody (N-term)

Cat. Num. AAA9217113

• Gene Names: LEO1; RDL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: Leo1-S151; Polyclonal Antibody; Leo1-S151 Antibody (N-term); RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein; LEO1; RDL; anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Leo1-S151 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the N-terminal region of human Leo1-S151.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 666

• Applicable Applications for anti-LEO1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:1000
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Nucleus.
• Tissue Location: Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
• Antigen Region: 129-158 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Leo1 Antibody (S151) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LEO1 antibody

Description: LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al. , 2005 [PubMed 15632063]).

Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.

Product Categories/Family for anti-LEO1 antibody

Primary Antibodies

References

Sugiyama, N., et al. Mol. Cell Proteomics 6 (6):1103-1109 (2007). Olsen, J.V., et al. Cell 127 (3):635-648 (2006). Olsen, J.V., et al. Cell 127 (3):635-648 (2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24 (10):1285-1292 (2006). Mosimann, C., et al. Cell 125 (2):327-341 (2006). Zhu, B., et al. Mol. Cell 20 (4):601-611 (2005). Zhao, L., et al. FASEB J. 19 (6):521-532 (2005). Rozenblatt-Rosen, O., et al. Mol. Cell. Biol. 25 (2):612-620 (2005). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (33):12130-12135 (2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (33):12130-12135 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 20270337
• NCBI GeneID: 123169
• NCBI Accession #: NP_620147.1
• NCBI GenBank Nucleotide #: NM_138792.3
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 75404 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 1
• NCBI Official Synonym Full Names: LEO1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN

NCBI Description

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: centrosome; nucleolus; nucleoplasm

Molecular Function: protein binding

Biological Process: endodermal cell fate commitment; histone H2B ubiquitination; histone monoubiquitination; mRNA polyadenylation; negative regulation of myeloid cell differentiation; positive regulation of mRNA 3'-end processing; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; stem cell maintenance; Wnt receptor signaling pathway