Western Blot (WB)
(Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human SPRED1 Polyclonal Antibody | anti-SPRED1 antibody
SPRED1 Antibody (C-term)
Western Blot (WB)
(Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Szafranski,K., Genome Biol. 8 (8), R154 (2007)
Yoshida,T., Oncogene 25 (45), 6056-6066 (2006)
King,J.A., Biochem. J. 388 (PT 2), 445-454 (2005)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]
Uniprot Description
SPRED1: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Defects in SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS). It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 15q14
Cellular Component: nucleoplasm; cytoplasm; caveola
Molecular Function: protein serine/threonine kinase inhibitor activity; protein binding; stem cell factor receptor binding; protein kinase binding; phosphatase binding
Biological Process: multicellular organismal development; positive regulation of DNA damage response, signal transduction by p53 class mediator; inactivation of MAPK activity
Disease: Legius Syndrome
Research Articles on SPRED1
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Product Notes
The SPRED1 spred1 (Catalog #AAA9202313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 302-331. The SPRED1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPRED1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the SPRED1 spred1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPRED1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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