EDA sirna

EDA siRNA (Mouse)

Cat. Num. AAA8211276

• Gene Names: Eda; Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EDA; EDA siRNA (Mouse); ED1; TA; Ectodysplasin-A; EDA protein homolog; Tabby protein; EDA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EDA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 377

• Applicable Applications for EDA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EDA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EDA sirna

siRNA to inhibit EDA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295789018
• NCBI GeneID: 13607
• NCBI Accession #: NP_001171408.1
• NCBI GenBank Nucleotide #: NM_001177937.1
• UniProt Accession #: O54693; O35705; Q9QWJ8; Q9QZ01; Q9QZ02; B7ZMT7
• Molecular Weight: 39,988 Da
• NCBI Official Full Name: ectodysplasin-A isoform 2
• NCBI Official Synonym Full Names: ectodysplasin-A
• NCBI Official Symbol: Eda
• NCBI Official Synonym Symbols: Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• NCBI Protein Information: ectodysplasin-A
• UniProt Protein Name: Ectodysplasin-A
• Protein Family: Ectodysplasin
• UniProt Gene Name: Eda
• UniProt Synonym Gene Names: Ed1; Ta
• UniProt Entry Name: EDA_MOUSE

Uniprot Description

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Cellular Component: endoplasmic reticulum membrane; collagen; membrane; apical part of cell; integral to plasma membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: tumor necrosis factor receptor binding

Biological Process: skin development; positive regulation of I-kappaB kinase/NF-kappaB cascade; pigmentation; hair follicle development; multicellular organismal development; cell-matrix adhesion; immune response; gene expression; positive regulation of NF-kappaB import into nucleus; cell differentiation; odontogenesis of dentine-containing teeth