Rabbit anti-Human Urotensin II Polyclonal Antibody | anti-UII antibody

Urotensin II, NT (UII) (MaxLight 650)

Cat. Num. AAA6504922

• Gene Names: UTS2; UII; U-II; UCN2; PRO1068
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Urotensin II; Polyclonal Antibody; NT (UII) (MaxLight 650); anti-UII antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UTS2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 124

• Applicable Applications for anti-UII antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human UTS2 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UTS2 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the UTS2 antibody detected the UTS2 protein (arrow).)

Immunohistochemistry (IHC)

(UTS2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UTS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(UTS2 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-UII antibody

Antibodies; Urotensin

References

1. Brailoiu, E., Peptides 29(5), 721-726 (2008). 2. Cirillo, P., J. Thromb. Haemost. 6(5), 726-736 (2008). 3. Zoccali, C.Hypertension 51(2), 326-333 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5803209
• NCBI GeneID: 10911
• NCBI Accession #: NP_006777
• NCBI GenBank Nucleotide #: NP_006777.1
• UniProt Accession #: O95399
• Molecular Weight: 14.314
• NCBI Official Full Name: urotensin-2 isoform b preproprotein
• NCBI Official Synonym Full Names: urotensin 2
• NCBI Official Symbol: UTS2
• NCBI Official Synonym Symbols: UII; U-II; UCN2; PRO1068
• NCBI Protein Information: urotensin-2
• UniProt Protein Name: Urotensin-2
• Protein Family: Urotensin
• UniProt Gene Name: UTS2
• UniProt Synonym Gene Names: U-II; UII
• UniProt Entry Name: UTS2_HUMAN

NCBI Description

This gene encodes a mature peptide that is an active cyclic heptapeptide absolutely conserved from lamprey to human. The active peptide acts as a vasoconstrictor and is expressed only in brain tissue. Despite the gene family name similarity, this gene is not homologous to urocortin, a member of the sauvagine/corticotropin-releasing factor/urotensin I family. Most of the proprotein is cleaved to make the mature peptide. Transcript variants encoding different preproprotein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UTS2: Highly potent vasoconstrictor. Belongs to the urotensin-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: response to drug; positive regulation of synaptic transmission, cholinergic; positive regulation of circadian sleep/wake cycle, REM sleep; negative regulation of heart rate; negative regulation of glomerular filtration; positive regulation of collagen biosynthetic process; positive regulation of heart rate; response to testosterone stimulus; positive regulation of vasodilation; negative regulation of insulin secretion; synaptic transmission; positive regulation of fibroblast proliferation; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; muscle contraction; regulation of blood pressure; response to hypoxia; negative regulation of blood pressure; positive regulation of cell differentiation; positive regulation of blood pressure