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Typical Testing Data

Rat Endocrine Gland Vascular Endothelial Growth Factor ELISA Kit | EG-VEGF elisa kit

Rat Endocrine Gland Vascular Endothelial Growth Factor ELISA Kit

Gene Names
PROK1; PK1; PRK1; EGVEGF
Reactivity
Rat
Synonyms
Endocrine Gland Vascular Endothelial Growth Factor; Rat Endocrine Gland Vascular Endothelial Growth Factor ELISA Kit; EG-VEGF/PROK1/Prokineticin 1/EG-VEGF/EGVEGFblack mamba toxin-related protein/Endocrine-gland-derived vascular endothelial growth factor/Mambakine/PK1mambakine/PRK1prokineticin-1/PROK1/prokineticin 1; EG-VEGF elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
105
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for EG-VEGF elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat EG-VEGF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat EG-VEGF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat EG-VEGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat EG-VEGF can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
11,715 Da
NCBI Official Full Name
EG-VEGF
NCBI Official Synonym Full Names
prokineticin 1
NCBI Official Symbol
PROK1
NCBI Official Synonym Symbols
PK1; PRK1; EGVEGF
NCBI Protein Information
prokineticin-1
UniProt Protein Name
Prokineticin-1
UniProt Gene Name
PROK1
UniProt Synonym Gene Names
EG-VEGF
UniProt Entry Name
PROK1_HUMAN

NCBI Description

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Uniprot Description

PROK1: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Belongs to the AVIT (prokineticin) family.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding; growth factor activity

Biological Process: activation of MAPK activity; angiogenesis; circadian rhythm; positive regulation of cell division; positive regulation of cell proliferation; regulation of angiogenesis

Research Articles on EG-VEGF

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Product Notes

The Rat EG-VEGF prok1 (Catalog #AAA761387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA761387 ELISA Kit recognizes Rat EG-VEGF. It is sometimes possible for the material contained within the vial of "Endocrine Gland Vascular Endothelial Growth Factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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