Mouse ectodysplasin A ELISA Kit | EDA elisa kit

Mouse Ectodysplasin-A, EDA ELISA Kit

Cat. Num. AAA930835

• Gene Names: Eda; Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• Reactivity: Mouse
• Synonyms: ectodysplasin A; Mouse Ectodysplasin-A; EDA ELISA Kit; Mouse Ectodysplasin-A (EDA) ELISA kit; RP11-351K23.1; ED1; ED1-A1; ED1-A2; EDA1; EDA2; HED; XHED; XLHED; X-linked anhidroitic ectodermal dysplasia protein; EDA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse EDA. No significant cross-reactivity or interference between mouse EDA and analogues was observed.
• Sequence Length: 389

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml
• Sensitivity: Typically less than 3.9 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EDA elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 295789018
• NCBI GeneID: 13607
• NCBI Accession #: NP_001171408.1
• NCBI GenBank Nucleotide #: NM_001177937.1
• UniProt Accession #: O54693; O35705; Q9QWJ8; Q9QZ01; Q9QZ02; B7ZMT7
• Molecular Weight: 41,603 Da
• NCBI Official Full Name: ectodysplasin-A isoform 2
• NCBI Official Synonym Full Names: ectodysplasin-A
• NCBI Official Symbol: Eda
• NCBI Official Synonym Symbols: Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• NCBI Protein Information: ectodysplasin-A; EDA protein homolog
• UniProt Protein Name: Ectodysplasin-A
• Protein Family: Ectodysplasin
• UniProt Gene Name: Eda
• UniProt Synonym Gene Names: Ed1; Ta
• UniProt Entry Name: EDA_MOUSE

Uniprot Description

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Cellular Component: endoplasmic reticulum membrane; collagen; membrane; integral to plasma membrane; apical part of cell; integral to membrane; plasma membrane; extracellular region

Molecular Function: tumor necrosis factor receptor binding

Biological Process: skin development; positive regulation of I-kappaB kinase/NF-kappaB cascade; pigmentation; hair follicle development; cell-matrix adhesion; multicellular organismal development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; cell differentiation; odontogenesis of dentine-containing teeth