ALKBH1 sirna

ALKBH1 siRNA (Mouse)

Cat. Num. AAA8234248

• Gene Names: Alkbh1; Abh; alkB; hABH; Alkbh; 2700073G19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALKBH1; ALKBH1 siRNA (Mouse); ABH; ALKBH; Alkylated DNA repair protein alkB homolog 1; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1; ALKBH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ALKBH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 389

• Applicable Applications for ALKBH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALKBH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALKBH1 sirna

siRNA to inhibit ALKBH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156523266
• NCBI GeneID: 211064
• NCBI Accession #: NP_001096035.1
• NCBI GenBank Nucleotide #: NM_001102565.1
• UniProt Accession #: P0CB42
• Molecular Weight: 43,746 Da
• NCBI Official Full Name: alkylated DNA repair protein alkB homolog 1
• NCBI Official Synonym Full Names: alkB, alkylation repair homolog 1 (E. coli)
• NCBI Official Symbol: Alkbh1
• NCBI Official Synonym Symbols: Abh; alkB; hABH; Alkbh; 2700073G19Rik
• NCBI Protein Information: alkylated DNA repair protein alkB homolog 1
• UniProt Protein Name: Alkylated DNA repair protein alkB homolog 1
• Protein Family: DNA demethylase
• UniProt Gene Name: Alkbh1
• UniProt Synonym Gene Names: Abh; Alkbh
• UniProt Entry Name: ALKB1_MOUSE

Uniprot Description

ALKBH: Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. Belongs to the alkB family.

Protein type: EC 1.14.11.33; DNA repair, damage; EC 4.2.99.18

Cellular Component: mitochondrion

Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; ferrous iron binding; chemoattractant activity

Biological Process: developmental growth; DNA dealkylation; positive chemotaxis; in utero embryonic development; regulation of gene expression; neuron migration; RNA repair; negative regulation of neuron apoptosis; DNA repair; cell differentiation; DNA catabolic process, endonucleolytic; neurite development; placenta development