Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit | PTF1a elisa kit

Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit

Cat. Num. AAA2704642

• Gene Names: PTF1A; PACA; PAGEN2; bHLHa29; PTF1-p48
• Reactivity: Human
• Synonyms: Pancreas Specific Transcription Factor 1a (PTF1a); Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit; PTF1-A; PTF1-p48; Class A basic helix-loop-helix protein 29; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pancreas Specific Transcription Factor 1a (PTF1a).; No significant cross-reactivity or interference between Pancreas Specific Transcription Factor 1a (PTF1a) and analogues was observed.
• Sequence Length: 328

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreas Specific Transcription Factor 1a (PTF1a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreas Specific Transcription Factor 1a (PTF1a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTF1a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreas Specific Transcription Factor 1a (PTF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreas Specific Transcription Factor 1a (PTF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pancreas Specific Transcription Factor 1a (PTF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreas Specific Transcription Factor 1a (PTF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30039710
• NCBI GeneID: 256297
• NCBI Accession #: NP_835455.1
• NCBI GenBank Nucleotide #: NM_178161.2
• UniProt Accession #: Q7RTS3; Q9HC25
• Molecular Weight: 34,970 Da
• NCBI Official Full Name: pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: PTF1A
• NCBI Official Synonym Symbols: PACA; PAGEN2; bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• Protein Family: Pancreas transcription factor
• UniProt Gene Name: PTF1A
• UniProt Synonym Gene Names: BHLHA29; PTF1P48; bHLHa29; PTF1-p48

NCBI Description

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; protein dimerization activity

Biological Process: cerebellum development; embryonic development; exocrine pancreas development; neuron fate commitment; pancreas development; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; retinoic acid receptor signaling pathway; tissue development; transcription from RNA polymerase II promoter

Disease: Pancreatic Agenesis 2; Pancreatic And Cerebellar Agenesis