Western Blot (WB)
(Western Blot (WB) analysis of ALKBH1 polyclonal antibody)
Rabbit anti-Human ALKBH1 Polyclonal Antibody | anti-ALKBH1 antibody
ALKBH1 Polyclonal Antibody
Gene Names
ALKBH1; ABH; ABH1; alkB; hABH; ALKBH
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ALKBH1; Polyclonal Antibody; ALKBH1 Polyclonal Antibody; ABH; ABH1; alkB; AlkB; alkylation repair homolog 1 (E. coli); ALKB1_HUMAN; ALKBH; Alkylated DNA repair protein alkB homolog 1; Alkylation repair homolog 1; Alkylation repair; alkB homolog; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1; hABH; anti-ALKBH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ALKBH1 polyclonal antibody detects endogenous levels of ALKBH1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
389
Applicable Applications for anti-ALKBH1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB 1:500-1:2000
IP -1:50-1:200
IP -1:50-1:200
Immunogen
Recombinant full length Human ALKBH1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ALKBH1 polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of ALKBH1 polyclonal antibody)
Related Product Information for anti-ALKBH1 antibody
ALKB protects DNA and RNA against damage from methylating compounds from the environment by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzymes act by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate, and molecular oxygen as the oxidizing agent. Deficiencies in DNA and RNA repair in mammals are associated with cancer, neurological disease and developmental defects. ALKB plays a role in resistance to anti-cancer drugs which attempt to damage tumor DNA. Escherichia coli ALKB protein belongs to the superfamily of 2-oxoglutarate- and iron(II)-dependent oxygenases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 43kDa
NCBI Official Full Name
nucleic acid dioxygenase ALKBH1
NCBI Official Synonym Full Names
alkB homolog 1, histone H2A dioxygenase
NCBI Official Symbol
ALKBH1
NCBI Official Synonym Symbols
ABH; ABH1; alkB; hABH; ALKBH
NCBI Protein Information
nucleic acid dioxygenase ALKBH1
UniProt Protein Name
Alkylated DNA repair protein alkB homolog 1
Protein Family
UniProt Gene Name
ALKBH1
UniProt Synonym Gene Names
ABH; ABH1; ALKBH
UniProt Entry Name
ALKB1_HUMAN
NCBI Description
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008]
Research Articles on ALKBH1
Similar Products
Product Notes
The ALKBH1 alkbh1 (Catalog #AAA3011414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB 1:500-1:2000 IP -1:50-1:200. Researchers should empirically determine the suitability of the ALKBH1 alkbh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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