ZMIZ1 sirna

ZMIZ1 siRNA (Human)

Cat. Num. AAA8219234

• Gene Names: ZMIZ1; MIZ; RAI17; ZIMP10; hZIMP10; TRAFIP10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZMIZ1; ZMIZ1 siRNA (Human); KIAA1224; RAI17; ZIMP10; Zinc finger MIZ domain-containing protein 1; PIAS-like protein Zimp10; Retinoic acid-induced protein 17; ZMIZ1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZMIZ1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1067

• Applicable Applications for ZMIZ1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZMIZ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZMIZ1 sirna

siRNA to inhibit ZMIZ1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543543
• NCBI GeneID: 57178
• NCBI Accession #: NP_065071.1
• NCBI GenBank Nucleotide #: NM_020338.3
• UniProt Accession #: Q9ULJ6; Q5JSH9; Q7Z7E6
• Molecular Weight: 107,270 Da
• NCBI Official Full Name: zinc finger MIZ domain-containing protein 1
• NCBI Official Synonym Full Names: zinc finger, MIZ-type containing 1
• NCBI Official Symbol: ZMIZ1
• NCBI Official Synonym Symbols: MIZ; RAI17; ZIMP10; hZIMP10; TRAFIP10
• NCBI Protein Information: zinc finger MIZ domain-containing protein 1
• UniProt Protein Name: Zinc finger MIZ domain-containing protein 1
• Protein Family: Zinc finger MIZ domain-containing protein
• UniProt Gene Name: ZMIZ1
• UniProt Synonym Gene Names: KIAA1224; RAI17; ZIMP10
• UniProt Entry Name: ZMIZ1_HUMAN

NCBI Description

This gene encodes a member of the PIAS (protein inhibitor of activated STAT) family of proteins. The encoded protein regulates the activity of various transcription factors, including the androgen receptor, Smad3/4, and p53. The encoded protein may also play a role in sumoylation. A translocation between this locus on chromosome 10 and the protein tyrosine kinase ABL1 locus on chromosome 9 has been associated with acute lymphoblastic leukemia. [provided by RefSeq, Mar 2010]

Uniprot Description

RAI17: Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: zinc ion binding

Biological Process: heart morphogenesis; developmental growth; positive regulation of fibroblast proliferation; transcription, DNA-dependent; in utero embryonic development; vitellogenesis; cell aging; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis